黔西北蜜环菌的诱变选育与母种培养基筛选  

Mutation Breeding and Medium Screening of Armillaria mellea in Northwestern Guizhou

作　　者：杨清艳[1,2] 蔡传涛[1] 刘贵周[1] 文平 

机构地区：[1]中国科学院西双版纳热带植物园,云南昆明650223 [2]中国科学院大学,北京100049 [3]大方县九龙天麻开发有限公司,贵州大方551600

出　　处：《中国食用菌》2014年第3期12-15,共4页Edible Fungi of China

基　　金：国家科技支撑计划课题"喀斯特山区生态环境保护与植物类中药材开发关键技术研究与示范"(2007BAD53B04);贵州省科技厅毕节市人民政府中科院昆明分院科技合作项目"天麻栽培中替代菌材与野生萌发菌研究及其成果示范"(省地院合2011-5)

摘　　要：为解决黔西北蜜环菌菌种退化造成的天麻减产问题，首次对其进行诱变选育和母种培养基筛选，结果表明，接种菌索部位在尖端0～1．0cm时生长最好；氨水的诱变效果最好，1％氨水诱变2min获得稳定遗传的优良突变株；最适合优良突变株生长的培养基配方为葡萄糖20g、黄豆粉20g、马铃薯200g、琼脂15g、磷酸二氢钾2g，水1000mL,pH自然。In order to solve the problem of Gastrodia elata Blume＇ s yield drawdown caused by degradation of Armillaria mellea in northwestern Guizhou, mutation breeding and medium screening of Armillaria mellea were studied for the first time. The results suggested that Armillaria mellea＇ s growth was better when the It was proved that NH3 .H20 was the most effective mutagen. The best induced mutation conditions were found ： 1% NH3 . H2 0induced 2 min. The optimal culture medium for high -quality mutants was glucose 20 g, soybean meal 20 g, potato 200 g, agar 15 g, KH2PO4 2 g, H20 1 000 mL, natural pH.

关 键 词：蜜环菌 菌索 诱变选育 培养基筛选 

分 类 号：S646.1[农业科学—蔬菜学]