多重PCR快速检测甘蔗转基因成分研究  

Multiplex PCR for Rapid Detection of Components of Genetically Modified Sugarcane

作　　者：张卓[1,2] 许莉萍[1] 陈平华[1,2] 李林祥[3] 王恒波[1,2] 高世武[1] 施桂姣[1,2] 陈忠伟[1,2] 项慰 高三基[1] 刘迪[1,2] 邰连赛 陈容[1,2] 陈如凯[1] 

机构地区：[1]福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室,福建福州350002 [2]农业部甘蔗及制品质量监督检验测试中心转基因检测室,福建福州350002 [3]西南大学,重庆402460

出　　处：《热带作物学报》2014年第5期897-903,共7页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31070330);农业转基因生物安全技术检测项目(No.k42130004)

摘　　要：以单子叶植物常见外源转基因元件Ubiquitin启动子、NOS启动子、NOS终止子、Bar基因和Bt基因序列设计多重PCR引物,通过对PCR扩增体系中退火温度、Premix TaqTM浓度、引物浓度的优化,建立一种快速检测转基因甘蔗成分的多重PCR方法。结果表明:建立的体系一次能有效检测5个参数,其检测的最低DNA质量百分比可达1.0%,并在多个转基因甘蔗品种检测中得到验证。Primers were designed for multiplex PCR according to the sequences of Ubiquitin promoter, NOS promoter, NOS terminator, Bar and Bt genes, which are in most genetically modified monocotyledons. A rapid and effective multiplex PCR method was established to detect transgenic sugarcane through optimizing the annealing temperatures, concentrations of Premix TaqTM, primers and DNA of transgenic sugarcane in PCR programs. The multiplex PCR method was verified in detection of known transgenic sugarcane lines. The results showed the developed multiplex PCR method worked well with a limit of DNA of transgenic sugarcane down to 1.0%.

关 键 词：多重PCR 甘蔗 转基因检测 

分 类 号：S566.1[农业科学—作物学]