干旱胁迫对中粒种咖啡幼苗膜脂过氧化、抗氧化酶活性和渗透调节物质含量的影响  

Effects of Drought Stress on Lipid Peroxidation,Antioxidant Enzyme Activities and Osmolyte Content in Leaves of Coffea canephora Seedlings

作　　者：杨华庚[1] 颜速亮[1] 陈慧娟[1] 邓志声[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院科技信息研究所,海南儋州571737

出　　处：《热带作物学报》2014年第5期944-949,共6页Chinese Journal of Tropical Crops

摘　　要：以苗龄约8个月的中粒种咖啡袋育苗为材料．测定3种干旱胁迫强度下咖啡幼苗叶片的生理指标变化．旨在了解其对干旱胁迫的生理响应。结果表明：在轻度干旱胁迫下（土壤相对含水量为55％～60％）．与对照相比，中粒种咖啡幼苗叶片的可溶性蛋白、可溶性糖、脯氨酸含量、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性极显著增加．而丙二醛（MDA）含量和相对电导率没有显著变化。但在中度干旱或重度干旱胁迫下（土壤相对含水量为40％．45％或25％～30％），可溶性蛋白含量、SOD、POD、CAT活性显著或极显著减少，而可溶性糖、脯氨酸的积累减缓，MDA含量和相对电导率迅速上升。在适度干旱胁迫下，中粒种咖啡幼苗可通过提高抗氧化酶活性和渗透调节能力来增强其对干旱胁迫的耐性。Coffea canephora seedlings were used for the experiment at the age of about 8 months. Three levels of drought stress were applied in the present experiment, and the physiological indices of coffee leaves were measured to understand the physiological responses to drought stress. Experimental results showed that when coffee seedlings were exposed to mild drought stress （relative soil water content of 55% to 60%）, compared with the control group, soluble protein, soluble sugar and proline contents, SOD, POD and CAT activities increased significantly, while MDA contents and relative electrical conductivity had no significant change. But under moderate or severe drought stress（relative soil water content of 40% to 45% or 25% to 30%）, soluble protein content, SOD, POD and CAT activities reduced significantly, while soluble sugar and proline accumulated relatively slowly, and MDA content, relative electrical conductivity increase rapidly. These results show that Coffea canephora seedlings have improved drought tolerance by increasing antioxidant enzyme activities and osmoregulation ability under suitable drought stress conditions.

关 键 词：中粒种咖啡 干旱胁迫 抗氧化作用 渗透调节 膜脂过氧化 质膜透性 

分 类 号：S571.2[农业科学—作物学]