水杨酸结合超声波处理对芒果采后抗病性的影响  

Combination of Salicylic Acid and Ultrasound to Control Postharvest Anthracnose Caused by Colletotrichum gloeosporioides Penz. in Mango Fruit

作　　者：杨冬平[1,2] 高兆银[2] 李敏[2] 张正科[2] 陈亮[1,2] 杨波[1,2] 赵超[1] 胡美姣[2] 

机构地区：[1]海南大学环境与植物保护学院,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所农业部热带作物有害生物综合治理重点实验室海南省热带农业有害生物监测与控制重点实验室,海南海口571101

出　　处：《热带作物学报》2014年第5期974-979,共6页Chinese Journal of Tropical Crops

基　　金：公益性行业(农业)科研专项经费项目(No.201203092-02);海南大学热带生物资源教育部重点实验室青年基金(No.qnjj1230);海南大学211工程建设项目;中央级公益性科研院所基本科研业务费(No.2012hzs1J011;No.2013hzs1J012)

摘　　要：探讨水杨酸结合超声波处理对芒果采后炭疽病的抗病性及其相关防御酶活性的影响。以绿熟‘贵妃’芒为试验材料,用水杨酸(2 mmol/L)、超声波(40 kHz,500 W)单独处理及两者结合处理,贮藏24 h后进行损伤接种芒果炭疽菌(Colletotrichum gloeosporioides),并在接种后第6天测定病原菌的致病性;同时,对处理后未接种果实,每2 d取样一次,测定果肉组织中苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、几丁质酶(CHT)和β-1,3葡聚糖酶(GLU)等相关防御酶的活性和果皮中总酚含量的变化。结果表明:水杨酸能有效抑制芒果采后炭疽病病斑的扩展,能够提高相关防御酶的活性和果皮中总酚的积累;水杨酸结合超声波处理显著提高水杨酸单独处理的作用效果,显著增强了果实抗病性,是芒果采后炭疽病防控的新技术。The effect of salicylic acid（SA） combined with ultrasound（US） on anthracnose caused by C. gloeosporioides Penz. in harvested mango fruit （Mangifira indica cv. Guifei）was investigated. The fruit, treated with SA（2 mmol/L） or US（40 kHz, 500 W）alone or in combination,were inoculated with C. gloeosporioides after 24 h of treatment, and then stored at （25±1）℃. The pathogenicity of C. gloeosporioides was measured at 6 d after inoculation. Meanwhile, the content of total phenolic compounds and activities of phenylalanine ammonia lyase （PAL）, peroxidase（POD）, chitinase（CHT） and β-1, 3-glucanase（GLU） were investigated. The results showed that SA treatment was effective in inhibiting the development of anthracnose and increased the activities of defense-related enzymes such as PAL, POD, CHT, GLU and the content of total phenolic compounds, which were associated with higher disease resistance induced. The combined treatment was more effective than the SA treatment alone during storage. These results suggest that the combined treatment of SA and ultrasound may be a useful technique to reduce anthracnose in mango fruit.

关 键 词：芒果 水杨酸 超声波 抗病性 

分 类 号：Q945.8[农业科学—植物病理学]