甲硝唑在胆汁中的浓度动态分布的实验研究  

The Experimental Study of the Concentration and Dynamic Distribution of Metronidazole in Rabbit Bile

作　　者：郑惊雷[1] 梁力建[2] 王在国[1] 黄文静[3] 王雪丁[4] 

机构地区：[1]广东省东莞市人民医院外科,东莞523059 [2]中山大学附属第一医院肝胆外科,广州510080 [3]广东省药品检验所抗生素室,广州510180 [4]中山大学药学院,广州510080

出　　处：《国外医药（抗生素分册）》2014年第3期128-130,133,共4页World Notes on Antibiotics

摘　　要：目的研究甲硝唑在家兔胆汁中的浓度动态分布，为预防和治疗肝胆系统厌氧菌感染提供参考和依据。方法家兔行胆总管造瘘术，静脉注射甲硝唑后于不同时间点采集胆汁标本，采用高效液相色谱法（HPLC）测定其在胆汁中的浓度，了解用药后不同时间药物在胆汁中的动态分布。结果家兔静脉注射甲硝唑（40mg／kg）后0．25h胆药浓度即大大超过有效杀菌浓度，胆汁药物达峰时间Tmax为（0．29±0．10）h；峰浓度Cmax为（29．49±10．02）±g／mL，远远超过其对厌氧菌的有效杀菌浓度（0．25-2μg／mL），给药后4h胆药浓度仍达到最低抑菌浓度。结论甲硝唑可作为预防和治疗胆道厌氧菌感染较好的药物。Objective To study the concentration and dynamic distribution of metronidazole in rabbit bile. Methods After anaesthesia, the common bile duct of rabbit was isolated and cannulated with a silicone tube. The rabbits were administered intravenously with the dose of 40mg/kg of metronidazole. Bile（1.SmL） was collected at different time points after administration, and concentration of the antibiotic in bile was assayed by HPLC. The bile drug concentration-time data were gained. Results After a single i.v. administration of 40mg/kg of metronidazole in each rabbit, drug concentrations reached Cmax （29.49±10.02） μg/mL immediately in bile, which had largely exceeded the minimum inhibition concentrations （MIC） for most anaerobic organisms ranging from 0.25 to 2μg/mL. Then the bile concentration of metronidzole decreased with time. But even after 4 hours of the intravenous infusion, metronidazole was still to remain over the MIC. Conclusions Metronidazole could be recommended as a practical and promising antianaerobic drug for the treatment ofbiliary tract infection.

关 键 词：甲硝唑 厌氧菌感染 胆汁 动态分布 高效液相色谱法 

分 类 号：R978[医药卫生—药品]