饮水型砷暴露人群 ERβ mRNA 的表达及意义  

Expression and significance of ERβ mRNA of residents exposed to arsenic via drinking water

作　　者：韩晓红[1] 郭志伟[1] 杨卫红[2] 武克恭[1] 李艳红[1] 夏雅娟[1] 

机构地区：[1]内蒙古自治区地方病防治研究中心,呼和浩特010031 [2]山东省德州市疾病预防控制中心

出　　处：《卫生研究》2014年第3期472-476,共5页Journal of Hygiene Research

基　　金：国家自然科学基金(No.30960336);内蒙古自治区卫生厅项目(No.2010017)

摘　　要：目的测定不同饮水型砷暴露人群血液雌激素受体β(ERβ)mRNA表达水平,分析ERβmRNA表达水平与砷致心脏损伤的关系,进一步探讨砷的内分泌干扰效应。方法采用分子流行病学调查方法,在饮水型地方性砷中毒病区选择调查对象273人,依据饮水砷浓度分为高、中、低和对照4个剂量组,运用实时荧光定量RT-PCR技术,检测其血液ERβmRNA表达水平。结果人群ERβmRNA表达量随着饮用水砷浓度及尿砷含量的增加而上升(r分别为0.159和0.21,P<0.05);随水砷浓度的增加Q-Tc间期延长患病率上升(χ2=4.35,P=0.037),各组ERβmRNA表达水平与Q-Tc间期延长患病率变化趋势一致;Tp-Te间期随水砷浓度的增加而延长(r=0.199,P=0.023),同样也随ERβmRNA表达的增加而延长(r=0.205,P=0.019);各组心律失常患病率几乎与ERβmRNA表达水平变化趋势同步。结论长期砷暴露可潜在地影响人体ERβ基因表达,砷致Q-Tc间期和Tp-Te间期延长与干扰ERβ基因表达可能有密切联系。Objective To detect ERβ mRNA expression of subjects exposed to different arsenic drinking water, and to analyze the potential relationship between their abnormal expression and heart injury caused by arsenic in order to study the endocrine disturbing effect of arsenic. Methods Molecular epidemiological method was used. The study subjects included a total of 273 residents exposed to arsenic via drinking water, and they were divided into four groups according to arsenic concentration, and their blood ERβ mRNA expression was detected by real-time RT-PCR. Results The level of ERβ mRNA expression rose with increasing of water As and urine As（r =0. 159, 0.21, P 〈0.05）. The prevalence of Q-Tc interval prolongation aggravated with increasing of water As （X2 = 4.35, P =0. 037）, while according with the variation of ERβ mRNA expression. Tp-Te interval prolonged with increasing of water As（ r = 0. 199, P =0. 023 ）, as well prolongedwith increasing of ERβ mRNA expression （ r = 0. 205, P = 0. 019）. The prevalence of arrhythmia was almost according with the variation ofERβ mRNA expression. Conclusion chronic arsenic exposure can potentially disturb ERβ mRNA expression, as well as there are possible relationship between ERβ mRNA abnormal expression and Q-Tc interval prolongation and Tp-Te interval prolonged caused by arsenic.

关 键 词：砷 ERΒ MRNA 心脏损伤 

分 类 号：R994.6[医药卫生—毒理学] R123.5[医药卫生—药学]