整合素α4β7在肠道表达的意义  

Significance of integrin α4 β7 expression in intestine

作　　者：康伟芳[1] 蒋就喜[1] 胡婷婷[1] 王雪雯[1] 张帅[1] 孔衍琳 

机构地区：[1]桂林医学院传染病教研室,广西桂林541004

出　　处：《中国现代医学杂志》2014年第8期55-58,共4页China Journal of Modern Medicine

基　　金：广西本级技术研究与开发项目(No:桂财教[2010]124号;桂财教[2012]98号);广西医疗卫生重点科研课题(No:重2010055);桂林市科学研究与技术开发项目(No:市科[2010]37号);2013年校地校企共建科技创新平台立项建设项目(No:桂教科研市科[2013]8号)

摘　　要：目的通过对外周血和肠道组织CD3+CD4+T淋巴细胞整合素α4β7表达率的检测,从分子水平为肠道作为HIV感染的主要部位提供依据。方法采用流式细胞仪对外周血和肠道组织中CD3+CD4+T淋巴细胞整合素α4β7表达情况进行分析,并对数据进行统计学处理。结果外周血CD3+CD4+T淋巴细胞整合素α4β7表达率为(30.2±5.1)%,肠道组织CD3+CD4+T淋巴细胞整合素α4β7表达率为(45.2±6.2)%,差异有统计学意义(P<0.01)。结论整合素α4β7受体使CD3+CD4+T淋巴细胞向肠相关淋巴组织归巢,肠道组织CD3+CD4+T淋巴细胞整合素α4β7表达率高于外周血。肠道CD3+CD4+T淋巴细胞高表达于整合素α4β7,使肠道作为HIV感染的主要部位成为可能。[Objective] To provide evidence of molecular level of intestine as major site of HIV infection, we detected α4β7^＋ CD3^＋ CD4^＋ T lymphocytes expression rate in peripheral blood and intestinal tissue. [Methods] The expression of integfin α4β7^＋, CD3^＋ and CD4^＋ in T lymphocytes were tested by flow cytometry, hen data were analyzed statistically. [ Results] The expression rate of α4β7 CD3^＋ CD4^＋ T lymphocytes was （30.2 ±5.1）% in peripheral blood and （45.2 ±6,2）% in intestinal tissue respectively, it was significantly different between peripheral blood and intestinal tissue （P〈0.01）. [Conclusion] Integrin α4β7 makes CD3^＋ CD4^＋ T lymphocytes to the gut-associated lymphoid tissue, the expression rate ofα4β7^＋ CD3^＋ CD4^＋ T lymphocytes is higher in intestinal tissue than in peripheral blood. The high expression of integrin α4β7 in CD3^＋ CD4^＋ T lymphocytes makes intestinal tissue become maior site of HIV infection possibly.

关 键 词：整合素α4β7 流式细胞仪 肠道组织 人免疫缺陷病毒 

分 类 号：R593.32[医药卫生—内科学] R446.62[医药卫生—临床医学]