脉冲电磁场对小鼠成骨细胞归巢基因的作用  

Effects of pulsed electromagnetic fields on homing genes of mouse osteoblasts

作　　者：杨福军[1,2] 孙元明[1,2] 徐文清[1,2] 赵洁[1,2] 王德芝[1,2] 樊飞跃[1,2] 

机构地区：[1]中国医学科学院北京协和医学院放射医学研究所,天津300192 [2]天津市放射医学与分子核医学重点实验室

出　　处：《国际生物医学工程杂志》2014年第2期81-84,共4页International Journal of Biomedical Engineering

基　　金：国家自然科学基金面上项目（81273005）;天津市自然科学基金资助项目（12JCYBJC16300）;北京协和医学院研究生创新基金项目（2010-1001-005）

摘　　要：目的 研究脉冲电磁场（PEMF）对小鼠成骨细胞上与造血干细胞（HSC）归巢、增殖有关基因的作用.方法 9周龄C57BL/6J小鼠,采用频率70 Hz、电磁场强度2 mT的PEMF作用4～5周,每周作用6d,对照组小鼠不接受PEMF作用.作用结束后,取双侧股骨和胫骨,胶原酶消化、分离细胞,再经磁珠和流式分选、收集ALCAM＋Sca-1-成骨细胞,最后通过表达谱芯片和定量PCR检测ALCAM＋Sca-1-成骨细胞上与HSC归巢、增殖有关的基因.结果 PEMF作用下,成骨细胞上Jagl（其对应HSC上的受体为Notch）和Ang-1（其对应HSC上的受体为Tie-2）的表达增强.结论 PEMF可能通过对ALCAM＋Sca-1-成骨细胞上与HSC归巢和自我更新有关基因的作用,调控HSC.Objective To investigate the effects of pulsed electromagnetic fields （PEMF） on homing and proliferation-related genes of mouse osteoblasts.Methods 9 week-old C57BL/6 mice were treated with PEMF （70 Hz,1 mT） for 4～5 weeks,while mice in control group didn＇t not receive PEMF.Bone marrow cells of femurs and tibias were flushed out,and the bones were minced and incubated at 37 ℃ with a type Ⅰ collagenase.Bone associated mononuclear cells （MNCs） were isolated via density centrifugation with Lymphoprep.Magnetic cell sorting was used before flow-cytometric sorting,and the ALCAM＋Sca-1-cells were collected.The homing and proliferation-related genes expressed in ALCAM＋Sca-1-cells were detected with high throughput microarray and RT-PCR.Results The expression of Jag1 and Ang-1 in mouse osteoblasts increased under the effects of PEMF.Conclusions PEMF may have regulation effects on HSC （hematopoietic stem cell） survival through modulating the homing and proliferationrelated genes in ALCAM＋Sca-1-osteoblasts.

关 键 词：脉冲电磁场 成骨细胞 干细胞龛 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]