狗枣猕猴桃叶片离体培养的研究  

Study onin vitro Culture of Actinidia kolomikta Leaves

作　　者：张玉杰[1] 杨忠 顾德峰[1] 

机构地区：[1]吉林农业大学园艺学院,吉林长春130118 [2]德惠市农业技术宣传教育中心,吉林德惠130300

出　　处：《北方园艺》2014年第10期97-101,共5页Northern Horticulture

摘　　要：以狗枣猕猴桃叶片为外植体,研究了影响狗枣猕猴桃愈伤组织诱导、分化、试管苗生根的主要因素。结果表明:狗枣猕猴桃叶片诱导愈伤组织的最佳培养基为MS+ZT 1.0mg/L+NAA 0.2mg/L,诱导率达到91.67%;不定芽分化的最佳培养基为MS+ZT 2.0mg/L+IBA0.2mg/L,分化率为89.45%,平均出芽个数为8.11个;狗枣猕猴桃极易生根,在以添加不同质量浓度的IAA、IBA和NAA的1/2MS基本培养基上,生根率都很高,多数处理可达100%;当基质配比V(田园土)∶V(草炭)∶V(沙子)=1∶1∶1时,试管苗的成活率为92%。Effect of different combinations of plant growth regulators on callus induction, differentiation and rooting of the test tube seedlings were studied by using leaves of Actinidia kolomikta as explants. The results showed that the optimal medium for callus inducement from leaf was MS＋ZT 1.0 rng/L＋NAA 0. 2 rng/L,and callus inducement rate reached 91.67 percent;the best medium for differentiation of adventitious buds was MS＋ZT 2. 0 m4g/L＋IBA 0.2 mg/L,and the differentiation rate was 89. 45 percent with an average number of 8. 11 budding. Actinidia kolomikta was extremely easy to rooting,and to add different concentrations of IAA,IBA and NAA on 1/2MS medium,rooting rate were very high,and most even achieved 100 percent;as matrix consisted of garden soil,peat and sand by a volume ratio of 1 ： 1 ： 1 cultures the test tube seedlings,the survival rate was 92 percent.

关 键 词：狗枣猕猴桃 叶片 离体培养 

分 类 号：S663.4[农业科学—果树学]