Ma Huang Tang ameliorates asthma though modulation of Th1/Th2 cytokines and inhibition of Th17 cells in ovalbumin-sensitized mice  

作　　者：MA Chun-Hua MA Zhan-Qiang FU Qiang MA Shi-Ping 

机构地区：[1]Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University

出　　处：《Chinese Journal of Natural Medicines》2014年第5期361-366,共6页中国天然药物（英文版）

基　　金：supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions

摘　　要：AIM： Ma Huang Tang （Ephedra decoction, MHT） is a famous classical formula from Shang Han Lun by Zhang Zhongjing in the Han Dynasty. The anti-asthmatic effects of MHT and the possible mechanisms were tested. METHOD： An asthma model was established by ovalbumin （OVA）-induction in mice. A total of forty-eight mice were randomly assigned to six experimental groups： control, model, dexamethasone （2 mg·kg^-1） and MHT （5, 10, and 20 mg·kg^-1）. Airway resistance （Raw） was measured by the forced oscillation technique, histological studies were evaluated by hematoxylin and eosin （HE） staining, Thl/Th2 and Th17 cytokines were evaluated by enzyme-linked immunosorbent assay （ELISA）, and Thl7 cells were evaluated by flow cytometry （FCM）.RESULTS： This study demonstrated that MHT inhibited OVA-induced increases in Raw and eosinophil cotmt; interleukin （IL）-4 and IL-17 levels were recovered in bronchoalveolar lavage fluid, increased IFN-γ level in bronchoalveolar lavage fluid. Histological studies demonstrated that MHT substantially inhibited OVA-induced eosinophilia in lung tissue. Flow cytometry studies demonstrated that MHT substantially inhibited Thl 7 cells.CONCLUSION： These findings suggest that MHT may effectively ameliorate the progression of asthma, and could be further investigated for potential use as a therapy for patients with allergic asthma,AIM: Ma Huang Tang(Ephedra decoction, MHT) is a famous classical formula from Shang Han Lun by Zhang Zhongjing in the Han Dynasty. The anti-asthmatic effects of MHT and the possible mechanisms were tested. METHOD: An asthma model was established by ovalbumin(OVA)-induction in mice. A total of forty-eight mice were randomly assigned to six experimental groups: control, model, dexamethasone(2 mg·kg-1) and MHT(5, 10, and 20 mg·kg-1). Airway resistance(Raw) was measured by the forced oscillation technique, histological studies were evaluated by hematoxylin and eosin(HE) staining, Th1/Th2 and Th17 cytokines were evaluated by enzyme-linked immunosorbent assay(ELISA), and Th17 cells were evaluated by flow cytometry(FCM). RESULTS: This study demonstrated that MHT inhibited OVA-induced increases in Raw and eosinophil count; interleukin(IL)-4 and IL-17 levels were recovered in bronchoalveolar lavage fluid, increased IFN-γ level in bronchoalveolar lavage fluid. Histological studies demonstrated that MHT substantially inhibited OVA-induced eosinophilia in lung tissue. Flow cytometry studies demonstrated that MHT substantially inhibited Th17 cells. CONCLUSION: These findings suggest that MHT may effectively ameliorate the progression of asthma, and could be further investigated for potential use as a therapy for patients with allergic asthma.

关 键 词：Ma Huang Tang ASTHMA THL/TH2 Thl7 cells 

分 类 号：R965[医药卫生—药理学]