A new flavonol C-glycoside and a rare bioactive lignanamide from Piper wallichii Miq. Hand.-Mazz  

作　　者：WANG Pei-Pei ZHAO Guo-Wei XIA Wen HAN En-Ji XIANG Lan 

机构地区：[1]School of Pharmaceutical Sciences, Shandong University [2]Qilu Hospital, Shandong University

出　　处：《Chinese Journal of Natural Medicines》2014年第5期377-381,共5页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.30271647,81073005);Shandong Province Young and Middle-Aged Scientists Research Awards Fund(No.BS2010YY032);Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry in China(No.42)

摘　　要：This study was conducted to investigate the chemical constituents of Piper wallichii （Miq.） Hand.-Mazz. and evaluate their biological activity. Compounds were isolated by various column chromatographic methods, and their structures were elucidated on the basis of physical characteristics and spectral data. The 1, 1-diphenyl-2-picrylhydrazyl （DPPH）-scavenging activity and acetylcholinesterase （AChE）-inhibitory activity of the compounds were evaluated. Five compounds were obtained and identified as 8-C-β-D-glucopyranosylkaempferol-3-O-β-D-glucopyranoside （1）, 1, 2-dihydro-6,8-dimethoxy-7-hydroxy-l-（3, 5-dimethoxy-4-hydroxyphenyl）-N^1, N^2-bis-[2-（4-hydroxyphenyl）ethyl]-2, 3-naphthalene dicarboxamide （2）, goniothalactam （3）, aristololactam A IIla （4） and piperlonguminine （5）. Compound 1 was a new flavonol C-glycoside, 2 was a rare lignanamide, which was isolated from the family Piperaceae for the first time, and compound 3 was isolated from this plant for the first time. Among them, 2 showed potent DPPH-scavenging activity, with IC50 of 31.38 ± 0.97 μmol·L^-1; Compounds 2, 3, and 4 showed AChE inhibitory activity at 100 μmol·L^-1, with inhibition rates of 28.57% ± 1.47%, 18.48% ± 2.41% and 17.4% ±3.03%, respectively.This study was conducted to investigate the chemical constituents of Piper wallichii(Miq.) Hand.-Mazz. and evaluate their biological activity. Compounds were isolated by various column chromatographic methods, and their structures were elucidated on the basis of physical characteristics and spectral data. The 1, 1-diphenyl-2-picrylhydrazyl(DPPH)-scavenging activity and acetylcholinesterase(AChE)-inhibitory activity of the compounds were evaluated. Five compounds were obtained and identified as 8-C-β-D-glucopyranosylkaempferol-3-O-β-D-glucopyranoside(1), 1, 2-dihydro-6,8-dimethoxy-7-hydroxy-1-(3, 5-dimethoxy-4-hydroxyphenyl)-N1, N2-bis-[2-(4-hydroxyphenyl)ethyl]-2, 3-naphthalene dicarboxamide(2), goniothalactam(3), aristololactam A IIIa(4) and piperlonguminine(5). Compound 1 was a new flavonol C-glycoside, 2 was a rare lignanamide, which was isolated from the family Piperaceae for the first time, and compound 3 was isolated from this plant for the first time. Among them, 2 showed potent DPPH-scavenging activity, with IC50 of 31.38 ± 0.97 μmol·L-1; Compounds 2, 3, and 4 showed AChE inhibitory activity at 100 μmol·L-1, with inhibition rates of 28.57% ± 1.47%, 18.48% ± 2.41% and 17.4% ± 3.03%, respectively.

关 键 词：Piper wallichii Flavonol C-glycoside Lignanamide DPPH scavenging activity AChE inhibitory activity 

分 类 号：R284.1[医药卫生—中药学]