高山离子芥CbCBF1基因耐盐性功能的初步研究  

The Preliminary Functional Research on the Salt Tolerance of CbCBF1

作　　者：贺泂杰[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050

出　　处：《安徽农业科学》2014年第14期4175-4175,4178,共2页Journal of Anhui Agricultural Sciences

基　　金：国家转基因生物新品种培育科技重大专项

摘　　要：[目的]研究高山离子芥CbCBF1基因的耐盐性功能。[方法]将高山离子芥的愈伤组织在高盐条件下进行时间梯度处理后,采用Real time PCR和Western blot分析CbCBF1在盐胁迫下转录和翻译水平表达量的变化。[结果]CbCBF1响应高盐胁迫,并随着处理时间的不同表达量呈现不同的变化趋势。[结论]该方法考察了高山离子芥CbCBF1基因的耐盐性功能,为高山离子芥CbCBF1基因的的进一步开发利用提供了依据。[Objective] To study salt tolerance function of CbCBF1.[Method] Time gradient processing was taken on the callus of CbCBF1 in extreme condition of high salt.Then the change of transcription expression of CbCBF1 was analyzed under the salt stress by Real time PCR and Western blot.[Result] It was found that CbCBF1 response to salt stress,and the express of CbCBF1 present different trends under the different processing time.[Conclusion] The salt tolerance function of CbCBF1 was investigated,which will provide reference for further development and utilization of CbCBF1.

关 键 词：CbCBF1 盐胁迫 表达 

分 类 号：S188[农业科学—农业基础科学]