4个多用途萱草品种离体快繁体系的建立  

Studies on Tissue Culture and Rapid Propagation of the Four Multi-role Hemerocallis fulva

作　　者：储博彦[1,2] 张全锋[1,2] 高超利 李金霞[1,2] 赵玉芬[1,2] 

机构地区：[1]河北省林业科学研究院,河北石家庄050061 [2]河北省林木良种工程技术研究中心,河北石家庄050061 [3]河北农业大学园林与旅游学院,河北保定071000

出　　处：《安徽农业科学》2014年第14期4189-4191,4214,共4页Journal of Anhui Agricultural Sciences

基　　金：石家庄市科技支撑计划课题"多用途萱草优良品种引选及栽培技术研究"(项目编号:11152052A)

摘　　要：[目的]建立4个多用途萱草品种的离体快繁体系。[方法]以4个多用途萱草品种为试材,研究消毒时间对不同外植体的影响,以及不同激素配比的培养基对愈伤组织、增殖培养和生根培养的影响。[结果]消毒时间以10 min最佳,花茎是萱草组织培养的最佳外植体;愈伤组织诱导的最佳培养基为:MS+1.5 mg/L 6-BA+0.2 mg/L IBA;在增殖培养中,MS+1.5 mg/L 6-BA+0.2 mg/L IBA为最佳增殖培养基。1/2MS+0.3 mg/L IBA和1/2MS+0.3 mg/L NAA培养基为4个品种的最佳生根培养基。[结论]该方法建立了4个多用途萱草品种的离体快繁体系,为萱草的种质资源栽培提供了理论依据。[Objective] To establish tissue culture and rapid propagation of the four multi-role Hemerocallis fulva.[Method] With the four multi-role H.fulva as test materials,effects of disinfection time on different explants were studied,effects of culture medium with different hormone ration on tissue callus,proliferation and rooting culture were studied.[Result] The results showed that 10 min was the best disinfection time,and the best explant was scape; The best medium for callus was MS ＋ 1.5 mg/L6-BA ＋ 0.2 mg/L IBA ; Shoot reproduction medium was MS ＋ 1.5 mg/L6-BA ＋ 0.2 mg/L IBA ; The best rooting medium was 1/2MS ＋ 0.3 mg/L IBA and 1/2MS ＋ 0.3 mg/L NAA.[Conclusion] The tissue cuhure and rapid propagation of four multi-role Hemerocallis fulva were established,which will provide theoretical basis for germplasm resource cultivation of Hemerocallis fulva.

关 键 词：多用途萱草 组织培养 培养基 外植体 快繁体系 

分 类 号：S188[农业科学—农业基础科学]