高邮鸭GNRHR基因克隆及卵巢中mRNA表达分析  

GNRHR Gene Clone and Its mRNA Expression in Ovary of Gaoyou Duck

作　　者：陈文峰 朱文奇[2] 胡艳[2] 丁余荣 李慧芳[2] 

机构地区：[1]江苏腾达源农牧有限公司,江苏泰州225300 [2]江苏省家禽科学研究所,江苏扬州225125

出　　处：《中国家禽》2014年第8期14-17,共4页China Poultry

基　　金：江苏高校省级重点实验室开放研究课题(K12036);现代农业品种创新(CX(11)1030);江苏省科技支撑计划(BE2011329);扬州市科技攻关(YZ2010048;2012078)

摘　　要：为了探究GNRHR基因在双黄蛋形成过程中的作用,本研究以高邮鸭为试验素材,应用克隆测序技术获得了高邮鸭促性腺激素释放激素(GNRHR)基因的全序列,并分析该基因的结构;采用荧光定量技术检测258日龄和330日龄高、低产双黄蛋鸭卵巢组织GNRHR基因mRNA的表达量,并分析了两者之间的差异。结果表明,鸭GNRHR基因序列全长为1 944 bp,共有3个外显子和2个内含子;258日龄和330日龄,高产双黄蛋鸭卵巢GNRHR基因mRNA的表达量高于低产双黄蛋鸭的表达量(P<0.05)。In order to explore the effect of GNRHR gene in forming procession of double-yolked egg, Gaoyou duck was chosed as experimental material, full length of duck GNRHR was cloned and its structure was analyzed. The mRNA expressions of GNRHR in high and low double-yolked egg duck populations at 258 and 330 days of age were detected by Real-time PCR repectively and the difference between the two genes were anlyzed. The results showed that the full length of GNRHR gene was 1 944 bp and composed of three exons and two introns. The mRNA expression of GNRHR gene in ovary of high double-yolked egg duck population was significant higher than that of low double-yolked egg duck population （P〈0.05）.

关 键 词：高邮鸭 双黄蛋 GNRHR基因 

分 类 号：S814[农业科学—畜牧学]