人参黑斑病生防用内生拮抗菌分离鉴定及发酵浓缩液的性质  

Isolation and identification of endophytic antagonistic bacterium against Alternaria panax and characteristics of its fermentation concentrate

作　　者：贾斌[1,2] 赵贞丽[2] 沈国娟[1,2] 屈俊亭 李熙英[1,2] 

机构地区：[1]延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002 [2]延边大学农学院,吉林延吉133002

出　　处：《中国森林病虫》2014年第3期5-10,17,共7页Forest Pest and Disease

基　　金：吉林省教育厅重点项目(2011800-611010002);延边大学科技发展计划项目(200802)

摘　　要：采用平板稀释法在几种中药材中分离内生拮抗细菌,结果人参根中分离到的内生拮抗细菌菌株数较多.采用平板对峙法筛选高效拮抗菌菌株,其中菌株BJ33对人参黑斑病具有很强的抑菌活性,其抑制率达到了80％.根据BJ33菌株的形态和生理生化特征以及采用16S rDNA同源性分析方法鉴定为解淀粉芽孢杆菌Bacillus amyloliquefaciens.BJ33菌株及发酵浓缩液对供试的6种植物病原菌菌丝生长均具有较强的抑制作用;BJ33菌株发酵浓缩液在15 ～ 35℃和pH5～11均具有较强的抑菌活性;BJ33菌株发酵浓缩液对高温、紫外线以及蛋白酶等具有较强的稳定性.From several Chinese herbs, endophytic antagonistic bacterium were isolated by the plate dilution method;and from ginseng roots, much more strains of endophytic antagonistic bacterium were i- solated in comparison with other Chinese herbs. As a result of screening efficient antagonistic bacteria strains by laboratory confrontation-culture, the strain B J33 from ginseng roots revealed a strong antagonistic activity against Alternaria panax with an antifungal rate of 80%. According to the phenotypic property ,physiological and biochemical characteristics combined with 16S rDNA sequence analysis ,the Strain B J33 was identified as Bacillus amyloliquefaciens. Both Strain B J33 and its fermentation concentrate all exhibited strong inhibition effects on mycelium growth of six plant pathogens examined in this study. The fermentation concentrate of B J33 strain had strong antifungal activities under conditions with the temperature from 15℃ to 35℃ and the pH value from 5 to 11, and maintained stable antifungal activities under high temperature, ultraviolet radiation and protease treatment.

关 键 词：人参 黑斑病 内生菌 解淀粉芽孢杆菌 生物防治 

分 类 号：S763.15[农业科学—森林保护学]