猪化脓隐秘杆菌的分离鉴定及病原特性研究  

Isolation and Identification of Arcanobacterium Pyogenes of Swine and Studies on the Pathogeny Speciality

作　　者：张乐宜[1] 蔡汝健[1] 宋长绪[1] 

机构地区：[1]广东省农业科学院动物卫生研究所广东省兽医公共卫生公共实验室,广东广州510640

出　　处：《动物医学进展》2014年第5期44-49,共6页Progress In Veterinary Medicine

基　　金：广东省科技厅项目(2012A020200014和2010A040301010);广州市科技计划项目(12C14071615);广东省生猪产业体系项目(粤农2009-380);广东省农业科学院院长基金项目(201313)

摘　　要：从广东惠州某猪场患有心包炎和肺炎的病猪内脏器官分离到1株细菌，经形态学观察、生化特性分析，初步确定为化脓隐秘杆菌（Arcanobacterium pyogenes ）。利用 PCR 方法对16 S rRNA 基因以及毒力基因 plo、nanH、nanP、cbpA 基因进行扩增及序列测定。Blast 结果表明，该分离株的16 S rRNA 基因与GenBank 数据库中分离自野猪、牛、羊、林麝等动物的化脓隐秘杆菌的核苷酸序列同源性为91．6％～95．0％，证实该分离株为猪化脓隐秘杆菌（A．pyogenes）。毒力基因扩增出 plo、nanH、fimA 基因，未扩增出nanP、cbpA、fimC、fimG 基因。动物致病性试验显示该菌株具有较强的致病性。药敏试验结果显示，分离菌株对头孢唑啉、头孢拉定、头孢噻肟、新生霉素、阿莫西林、氨苄西林、万古霉素高度敏感，对强力霉素、恩诺沙星、阿米卡星、氧氟沙星中度敏感，对链霉素、磺胺异噁唑、四环素、庆大霉素、林可霉素、新诺明、诺氟沙星耐药。本研究为猪化脓隐秘杆菌的鉴定及其流行病学调查与防治提供了参考依据。A strain of bacteria was isolated from the organs of a pig with pericarditis and pneumonia which came from a herd in Huizhou of Guangdong province.This isolation was identified as Corynebacterium pyogenes （Arcanobacterium pyogenes ）preliminary after the analysis of morphological and biochemical. The 16 S rRNA genes and virulence genes as PLO,nanH,nanP,cbpA,fimA,fimG,fimC were amplified by PCR and sequenced.The results of Blast indicated that the nucleotide sequence homology of 16 S rRNA gene was 91.6%~95.0% between this isolation and other animals as wild boar,cattle,sheep,Lin She and so on.This confirmed that the isolation was Arcanobacterium pyogenes （A.pyogenes）.The virulence genes as PLO,nanH,fimA,were detected and the nanP,cbpA,fimC,fimG genes were not.In Animal pathogenicity test,3 weeks old Babl/c mice were infected with the bacteria which the final concentration was 6.0×10^9 cfu/mL,then died after 24 h,indicated that this strain was more pathogenicity.Drug sensi-tive test indicated that this strain was high sensitive in cefazolin,cefradine,cefotaxime,novobiocin,ampicil-lin,vancomycin,andmoderate sensitive in doxycycline,enrofloxacin,amikacin,ofloxacin,and resistant in streptomycin,sulfamethoxazole,tetracycline,gentamicin,clindamycin,sulfamethoxazole,norfloxacin.This research provides the reference of identification,epidemiological investigation and control of porcine Ar-canobacterium pyogenes.

关 键 词：猪 化脓隐秘杆菌 分离鉴定 病原 

分 类 号：S852.619[农业科学—基础兽医学]