线栓法与改良三氯化铁法致大鼠大脑中动脉闭塞模型的对比研究  

Comparative Study of Middle Cerebral Artery Occlusion Model Induced by Suture and Modified Ferric Chloride Method in Rats

作　　者：包玉龙[1] 朱竟赫 范英兰[1] 刘禾[1] 宋达夫[1] 李显华[1] 赵磊[1] 

机构地区：[1]辽宁省中医药研究院,辽宁沈阳110034

出　　处：《动物医学进展》2014年第5期68-71,共4页Progress In Veterinary Medicine

基　　金：辽宁省科技厅课题(2011408004)

摘　　要：为探索一种较稳定的大鼠大脑中动脉闭塞(MCAO)模型,通过神经功能缺损评分、TTC染色、脑含水量检测和病理观察等方法,对线栓法和改良三氯化铁法致大鼠大脑中动脉闭塞(MCAO)模型进行比较观察。结果表明,改良三氯化铁法致大鼠大脑中动脉闭塞(MCAO)模型组的神经功能缺损评分、脑梗死面积、脑含水量均高于线栓法模型组,两组之间有显著性差异。说明改良三氯化铁法致大鼠大脑中动脉闭塞(MCAO)模型在梗死范围的稳定性、脑水肿程度、梗死体积等方面都明显优于线栓组,使该模型推广成为可能。To acquire a more stable kind of middle cerebral artery occlusion （MCAO） rat model, The MCAO models induced by suture and modified ferric trichloride were compared and analyzed, through the method of neurological impairment scores, TTC staining, cerebral water content test and pathological observation. Results indicated that neurological impairment score, cerebral irffarct size, cerebral water content of MCAO model group induced by modified ferric trichloride, were higher than those of the model group induced by suture, and the difference was significant. It can be concluded that modified ferric trichloride method was easier to operate, with higher success rate, and more stable than suture method on cerebral edema, infarct volume and weight, so that the model can be promoted to use.

关 键 词：线栓 三氯化铁 大鼠 大脑中动脉 闭塞 脑栓塞模型 

分 类 号：S852.3[农业科学—基础兽医学]