两种不同土鳖虫肠道菌群总DNA提取方法的比较及ERIC-PCR指纹图谱分析  

Comparison of Two Methods for Total DNA Extraction and Analysis of Intestinal Microflora in Eupolyphagasinesis by ERIC-PCR Fingerprint

作　　者：苏双良[1] 王慧[1] 李洪萍[2] 白秀娟[1] 

机构地区：[1]东北农业大学动物科学技术学院,黑龙江哈尔滨150030 [2]吉林农业大学中药材学院,吉林长春130118

出　　处：《动物医学进展》2014年第5期85-89,共5页Progress In Veterinary Medicine

摘　　要：比较不同方法提取土鳖虫肠道菌群总DNA的差异,并对土鳖虫肠道菌群组成多样性进行分析。采用试剂盒法和CTAB法提取土鳖虫肠道菌群总DNA,检测其浓度和纯度,扩增16SDNA进行鉴定,并利用ERIC-PCR技术对土鳖虫肠道菌群多样性进行比较分析。结果显示,两种方法均能提取DNA,用于16SDNA的扩增和ERIC-PCR分析,可用于进行土鳖虫肠道菌群的分子生态研究,CTAB法更能反应土鳖虫肠道菌群的多样性。To screen a method that could provide a high quality DNA template and investigate the similarity and diversity of intestinal microflora in Eupolyphaga sinesis,TIANamp Stool DNA Kit method and CTAB method were used to extract total DNA from intestinal microflora of the Eupolyphaga sinesis.The DNA quality was compared based on the concentration,purity and 16 S DNA PCR products,and analysis of intestinal microflora was carried out by ERIC-PCR fingerprint.The results showed that total DNA ex-tracted by each of the two methods could be used to 16 S DNA amplification and ERIC-PCR fingerprint a-nalysis.The quality of total DNA extracted by the two methods was good enough to study molecular ecol-ogy of intestinal microflora.But the result of ERIC-PCR of DNA extracted by CTAB method showed more microflora diversities.

关 键 词：土鳖虫 肠道菌群 总DNA提取 ERIC-PCR 

分 类 号：S855.12[农业科学—临床兽医学]