中药益智仁盐炙对正常大鼠结肠水通道蛋白4影响研究  

Effect study of Fructus Alpiniae Oxyphyllae Stir-frying With Salt Water on AQP4 of Normal Rats Colon

作　　者：王瑾[1] 谭茂兰[1] 汪云伟[1] 黄勤挽[1] 

机构地区：[1]成都中医药大学,四川成都611137

出　　处：《亚太传统医药》2014年第10期10-12,共3页Asia-Pacific Traditional Medicine

基　　金：国家自然科学基金(81001639);教育部新教师基金(2010513120002)

摘　　要：目的:观察益智仁盐炙对正常大鼠结肠水通道蛋白4(AQP4)的影响,研究益智仁燥性及盐炙润燥的作用机制。方法:将SPF级SD大鼠分为正常动物组、生益智仁组、盐益智仁组、清水炒益智仁组、生益智仁加盐组及生益智仁加增液汤组,各组动物每天灌胃给药1次,连续28天。于第28天处死动物,剖取结肠组织,采用免疫组织化学检测结肠中AQP4的表达情况。结果:与正常动物组比较,生益智仁组结肠AQP4表达显著增加(P<0.01);与生益智组比较,盐益智仁组、清水炒益智仁组、生益智仁加盐组及生益智仁加增液汤组结肠AQP4表达均显著降低(P<0.05或P<0.01),差异均具有统计学意义。结论:益智仁能增加正常大鼠结肠AQP4表达,促进肠道水分重吸收,从而造成便秘,体现其燥性效应;而益智仁盐炙后能降低其引起的燥性效应,其作用机制与盐炙过程中的加热炒制和辅料食盐有关。Objective：To study the effect of Fructus Alpiniae Oxyphyllae （FAO） Stir-frying with Salt Water on AQP4 of normal rats colon, so as to study the mechanism of dry effects and salt-processed moistening-dryness of FAO stir-frying with salt water. Methods：The SPF rats were divided into 6 groups： normal group, crude FAO group, FAO stir-frying with salt water and water groups, crude FAO coupled with salt group, FAO and increased liquid soup combination group. All rats were orally administered once a day for 28 d. All rats were sacrificed on the 28th day, and dissected the colon, then detected the expression of AQP4 of colon by immunohistochemistry. Results：Compared with normal group, the expression of AQP4 of crude FAO group were increased sig- nificantly（P〈~0. 01）; compared with crude FAO group, the expression of AQP4 of FAO Stir-frying with salt water and water groups, crude FAO coupled with salt group, FAO and increased liquid soup combination group were decreased significantly（P〈0.05 or P〈0.01）. Conclusion： FAO can increase the expression of AQP4 of normal animal colon, to promote intestinal reabsorption of water, then lead to constipation and reflect its dry effects; However, FAO stir-frying with salt water can reduce its dry effects by FAO, the mechanism was related to stir-frying and salt in the process of stir-frying with salt water.

关 键 词：益智仁 盐炙 大鼠结肠 水通道蛋白4 

分 类 号：R283[医药卫生—中药学] R285.5[医药卫生—中医学]