γ-干扰素受体在雌性山羊星状神经节的表达  

Expression characteristic of IFNGR in stellate ganglion of female goats

作　　者：李强[1] 王志豪[1] 金秀芳[1] 徐永平[1] 郭晓[1] 陈文东[1] 董伟[1] 

机构地区：[1]西北农林科技大学,杨凌712100

出　　处：《中国免疫学杂志》2014年第5期604-608,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(31072184);陕西省农业推广项目(k332021309)

摘　　要：目的:为了解雌性山羊星状神经节是否具备接受γ-干扰素(Interferon-γ,IFN-γ)作用的条件,即是否有IFN-γ受体(Interferon-γreceptor,IFNGR)存在。方法:取雌性山羊星状神经节,用免疫组化SP法和PCR方法检测IFNGR在星状神经节的表达情况。结果:结果显示,在雌性山羊星状的神经元、支持细胞和神经纤维均有IFNGR免疫阳性产物分布,但主要分布于神经元细胞膜与细胞核,IFNGR在神经元胞体的相对表达量极显著高于非神经细胞结构(P<0.01)。在山羊星状神经节扩增到IFNGR1基因的cDNA片段全长376 bp,山羊IFNGR1基因与绵羊基因同源性最高(98%),其次为牛(97%)。结论:在雌性山羊星状神经节中IFNGR主要在交感节后神经元表达与分布,具备对IFN-γ刺激做出反应的条件,提示雌性山羊星状神经节可能作为IFN-γ对心血管免疫调节途径和自主神经对心血管调节的神经途径之间相互协调的关键点。Objective：To detect the existence of IFNGR in the stellate ganglion in female goats .Methods：The stellate ganglia were taken from female goats .The expression of IFNGR was detected by PCR method and immunohistochemical SP staining .Results：The results showed that：IFNGR immunoreactive substances were distributed in neurons , supporting cells and passing fiber , and mainly in the cytomembrane and nuclei of neurons .The relative expression of IFNGR was very significantly higher than that of non-neuronal cells （P〈0.01）.The sequence of IFNGR1 gene amplified by PCR method contained 376 bp, and IFNGR1 gene of goats exhibited the highest homology with sheep（98%）,followed by cattle（97%）.Conclusion：The results suggested that the IFNGR in the Stellate Gan-glion of female goats mainly expressed and located in sympathetic postganglionic neurons which were provided with the conditions for the role of IFN-γ, which implied that the Stellate Ganglion may act as the critical point to concert the immune regulation of IFN -γand neu-roregulation of autonomic nerve on cardiovascular system .

关 键 词：γ-干扰素受体 星状神经节 免疫组织化学SP法 山羊 

分 类 号：S852.4[农业科学—基础兽医学]