IL-29对胰蛋白酶诱导的肥大细胞PARs表达的调节作用  

Effect of IL-29 on trypsin-induced protease-activated receptor expression on P815 mast cells

作　　者：隋丽[1] 陈冬[1] 张慧云[2] 何韶衡[3] 

机构地区：[1]辽宁医学院附属第一医院耳鼻咽喉科,锦州121001 [2]海南医学院病理生理学教研室,海口571101 [3]辽宁医学院附属第一医院变态反应与临床免疫研究中心,锦州121001

出　　处：《中国免疫学杂志》2014年第5期609-612,622,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(81241135;81060250;81172836;81030054);辽宁省博士科研启动基金资助项目(20101063)

摘　　要：目的:检测白细胞介素29(Interleukin-29,IL-29)对胰蛋白酶引起的肥大细胞蛋白酶激活受体(Protease activated receptor,PAR)-1,2,3,4表达的调节作用。方法:P815肥大细胞培养后,用不同浓度的IL-29、胰蛋白酶单独或联合激发肥大细胞,在不同时间点收集激发细胞,用流式细胞术(FCM)及实时定量PCR检测P815肥大细胞蛋白酶激活受体的表达。结果:IL-29单独作用能够下调肥大细胞PAR-1蛋白及mRNA水平的表达,上调PAR-3、PAR-4 mRNA的表达,与对照组相比差异有统计学意义(P<0.05);以IL-29预处理肥大细胞后,IL-29对胰蛋白酶诱导的肥大细胞PAR-2、PAR-3、PAR-4表达起促进作用,与对照组相比差异具有统计学意义(P<0.05)。结论:IL-29能够调节胰蛋白酶引起的肥大细胞PARs表达,从而参与肥大细胞相关的炎症反应。Objective：To investigate the modulatory effect of IL-29 on trypsin-induced protease activated receptors （PARs） ex-pression on P815 mast cell.Methods：After P815 mast cells were challenged with different concentrations of IL-29 alone or combined with trypsin for 2 h, 6 h and 16 h, the challenged cells were collected and analysed by flow cytometry to detect the protein expression of PARs on P815 cells, and analysed by real time RT-PCR to detect the mRNA expression of PARs on P 815 cells.Results：Compared with the corresponding control , IL-29 induced significantly decreased expression of PAR-1 at protein and mRNA level on P815 cells, and upregulated PAR-3, PAR-4 mRNA level on P815 cells, whereas IL-29 did little effect on the expressions of PAR-2,3,4 at protein level on P815 cells accordingly.Preincubation of mast cell with IL-29 did not alter trypsin-induced PAR-1 expression on P815 cells, whereas up-regulated expression of PAR-2, 3, 4 were detected when P815 cell were pre-treated with IL-29 before being challenged with trypsin compared with the corresponding control .Conclusion： IL-29 can upregulate trypsin-induced PARs expression on mast cells through which participated in mast cell related inflammation .

关 键 词：肥大细胞 白细胞介素29(IL-29) 胰蛋白酶 蛋白酶激活受体(PARs) 流式细胞术 

分 类 号：R329.8[医药卫生—人体解剖和组织胚胎学]