UPLC-MS法同时测定大鼠血浆中氧化芍药苷、没食子酸及咖啡酰基奎宁酸类成分  

Simultaneous determination of hydroxyl-paeoniflorin, gallic acid and dicaffeoylquinic acid in rat plasma by UPLC-MS

作　　者：郑林[1,2] 唐丽[1] 牟景丽[1] 黄勇[1,2] 董莉[1,2] 兰燕宇[1,2] 

机构地区：[1]贵阳医学院药学院,民族药与中药开发应用教育部工程研究中心,贵州贵阳550004 [2]贵州省药物制剂重点实验室,贵州贵阳550004

出　　处：《中成药》2014年第5期937-941,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81060335);贵州省社会发展攻关计划(黔科合SY字[2011]3033号);贵州省科学技术基金(黔科合J字[2012]2029号);贵州省中药现代化科技产业研究开发专项(黔科合中药字[2011]5081号)

摘　　要：目的建立UPLC-MS法同时测定大鼠静脉注射辛芍（灯盏细辛和赤芍）提取物后血浆中氧化芍药苷、没食子酸、1,3-二咖啡酰基奎宁酸、3,4-二咖啡酰基奎宁酸、4,5-二咖啡酰基奎宁酸5种成分的分析方法。方法血浆样品酸化后经乙腈沉淀除去蛋白,UPLC分析采用Waters Acquity BEH C18色谱柱,流动相为0.1%甲酸乙腈-0.1%甲酸水梯度洗脱,采用电喷雾电离源,扫描方式为选择性离子监测模式。结果 5种成分在血浆中线性关系良好,提取回收率91.95%～109.09%,日内、日间精密度和准确度良好,被测成分在大鼠体内消除较快。结论本分析方法特异、快速、准确灵敏,可用于氧化芍药苷、没食子酸、1,3-二咖啡酰基奎宁酸、3,4-二咖啡酰基奎宁酸、4,5-二咖啡酰基奎宁酸的药代动力学研究。AIM To develop a UPLC-MS method for simultaneously determing the contents of hydroxyl-paeoniflorin,gallic acid,1,3- dicaffeoylquinic acid,3,4- dicaffeoylquinic acid,and 4,5- dicaffeoylquinic acid after intravenous administration of Xinshao extract（ Erigerontis Herba,Paeoniae Radix rubra） in rat plasma. METHODS Following a protein precipitation with acidified acetonitrile for plasma samples. The UPLC analysis was performed on Waters Acquity BEH C18column and the mobile phase consisting of acetonitrile and water（ containing 0. 1% formic acid） in gradient elution manner. The compounds were ionized in the electrospray ionization ion source of the mass spectrometer and detected in the selective ion monitoring mode. RESULTS Good linearity was achieved for the five compounds,the extraction recovery was between 91. 95% to 109. 09,the intra-and inter-day precisions and the accuracy were good. The five compounds were eliminated in vivo rapidly. CONCLUSION The method is specific,sensitive and suitable for the pharmacokinetic studies of the five compounds.

关 键 词：UPLC-MS 药动学研究 辛芍提取物 大鼠 

分 类 号：R969.1[医药卫生—药理学]