血站核酸检测试剂的选择与应用  

Selection and application of nucleic acid testing (NAT) reagents in blood center

作　　者：李文[1] 李执如[2] 习燕[3] 李莹[1] 王欢[4] 赵欣[4] 

机构地区：[1]成都市血液中心质量管理科,四川成都610041 [2]成都市血液中心血液研究室,四川成都610041 [3]成都市血液中心器材设备科,四川成都610041 [4]成都市血液中心检验科,四川成都610041

出　　处：《现代预防医学》2014年第10期1868-1869,1887,共3页Modern Preventive Medicine

摘　　要：目的选择血站适用的核酸检测试剂并评估其应用效果。方法对5个厂家的试剂进行比选招标,选择进口和国产各1家试剂用于血液核酸检测。结果美国罗氏、上海科华核酸检测试剂为中标试剂。美国罗氏核酸检测试剂检测ELISA检测合格的血液标本75 638份,检出43例核酸阳性;上海科华核酸检测试剂检测ELISA检测合格的血液标本49 373份,检出4例核酸阳性。结论 2种中标核酸检测试剂的使用情况良好,满足预期要求。Objective The aim of this research was to select nucleic acid testing （NAT） reagents in blood center and to evaluate the effect. Methods Reagents from five manufacturers were compared. One foreign reagent and one domestic reagent were selected and used in nucleic acid test. Results Roche NAT reagent from the United States and Kehua NAT reagent from Shanghai were selected in this study. Roche NAT reagent was used to examine 75, 638 qualified blood samples tested by ELISA, and 43 samples were nucleic acid positive. Kehua NAT reagent was used to examine 49, 373 qualified blood samples tested by ELISA, and four samples were nucleic acid positive. Conclusion Both kinds of selected NAT reagents were useful and able to meet the expected requirements.

关 键 词：核酸检测 试剂 选择 应用 

分 类 号：R184[医药卫生—流行病学]