叶子花重瓣品种DOD基因序列比对分析  

Comparison of DOD gene sequence in multi-bracted cultivars of Bougainvillea

作　　者：苏进展 王想兰 冯世秀[3] 王晓明[3] 胡章立[4] 陈涛[3,4] 

机构地区：[1]厦门日懋城建园林建设股份有限公司,福建厦门361101 [2]深圳市日昇园林绿化有限公司,广东深圳518034 [3]深圳市中国科学院仙湖植物园,广东深圳518004 [4]深圳大学生命科学学院,广东深圳518060

出　　处：《草原与草坪》2014年第2期7-10,共4页Grassland and Turf

基　　金：深圳市科技创新委员会基础研究项目(JCYJ20120615172425764);深圳市城市管理局科研项目(200901)资助

摘　　要：叶子花品种的苞片色彩丰富艳丽,是研究颜色表达调控的优良材料。对调控甜菜色素合成酶的DOD基因进行比对分析,以探讨叶子花的DOD基因与其苞片颜色表达的关联。通过对5个具有不同苞片颜色的重瓣叶子花栽培品种DOD基因转录组测序,应用软件ClustalX 2.0进行DOD cDNA序列比对分析,结果显示其序列同源性达100%,其调控色素合成的功能基因序列并没有差异。叶子花重瓣栽培品种的不同苞片颜色的表达与DOD基因对色素合成的调控可能无直接关联。The cultivars of Bougainvillea are very rich in bract colors and good materials for studying colour expression.The paper studied the relationship between DOD gene and colour expression in the bracts of Bouga-invillea cultivars by analyzing DOD cDNA sequence that regulate the synthetase of betalain,The DOD cDNA of five Bougainvillea multi-bracted cultivars was cloned and sequenced respectively.The comparison of sequence indicated that there was no difference in DOD cDNA among the 5 cultivars with different bract colours.Therefore,it was preliminarily concluded that colour expression in the bracts of Bougainvillea cultivars is not directly correlated with the DOD gene regulating the pigment biosynthesis.

关 键 词：叶子花 重瓣栽培品种 甜菜色素 DOPA 4 5-双加氧酶 基因比对 

分 类 号：S685[农业科学—观赏园艺]