一氧化氮对铅胁迫下杂花苜蓿种子萌发及幼苗生理特性的影响  

Effects of nitric oxide on seed germination and seedling physiological response of Medicago variaunder Pb stress

作　　者：向华[1] 南丽丽[1] 李春晓[1] 王克婧 杜玺[1] 

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草原与草坪》2014年第2期77-80,85,共5页Grassland and Turf

基　　金：甘肃省高校基本科研业务费"一种植年限对苜蓿地土壤微生物多样性及群落结构的影响"项目资助

摘　　要：以杂花苜蓿阿尔冈金(Medicago varia cv.ALgonquin)为试验材料,采用沙培方法,研究了外源一氧化氮(NO)供体硝普钠(SNP)对Pb2+处理下杂花苜蓿种子萌发及幼苗生理特性的影响。结果表明,与对照比,用SNP(100μmol/L)、低Pb2+浓度(250mg/L)和SNP与低Pb2+浓度组合(100μmol/L SNP+250mg/L)处理均增加了杂花苜蓿种子的发芽势、发芽率、胚芽胚根长,提高了幼苗脯氨酸、可溶性糖及可溶性蛋白质的含量,增强了根系活力,降低了MDA含量。高Pb2+浓度(500mg/L)处理不利于杂花苜蓿种子萌发和幼苗生长,且100μmol/L SNP不能缓解高铅胁迫对种子萌发及幼苗生长的抑制作用。Medicago varia cv.Algonquin was used to study the effects of nitric oxide donor SNP on seed germination and seedlings physiological characteristics under lead stress by sand culture.The results showed that the treatments with SNP of 100 μmol/L concentration,Pb of 250 mg/L concentration,and Pb of 250 mg/L and SNP of 100 μmol/L concentrations resulted in the improvement of germination potential,germination rate, root length,shoot length and root activity,and increasing of proline,soluble sugar and soluble protein contents, but the MDA content was decreased.High concentration Pb solution （500 mg/L）inhibited seedling growth and SNP （100 μmol/L）could not reduce the damage in this case.

关 键 词：PB2+ 胁迫 杂花苜蓿 种子萌发 生理特性 

分 类 号：S332[农业科学—作物遗传育种]