益肾胶囊对糖尿病肾病大鼠肾组织TGF-β_1及Smad7表达的影响  

Effect of Yishen Capsule on the Expression TGF-β_1and Smad7 in Rats with Diabetic Nephropathy

作　　者：李慧[1] 方敬爱[1] 张晓东[1] 

机构地区：[1]山西医科大学第一医院肾内科,太原030001

出　　处：《中国中西医结合肾病杂志》2014年第3期194-196,I0001,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：国际科技合作项目(No.2011081066);国家自然基金资助项目(No.81173364)

摘　　要：目的:探讨益肾胶囊对糖尿病肾病大鼠肾组织TGF-β1及Smad7表达的影响。方法:将32只雄性Wistar大鼠,随机分为对照组、糖尿病肾病模型组、氯沙坦治疗组、益肾胶囊治疗组,每组8只。治疗组给予氯沙坦钾(20 mg·kg-1·d-1)、益肾胶囊(625 mg·kg-1·d-1)灌胃8周。测定血糖、24 h尿蛋白定量、血肌酐(Scr)和尿素氮(BUN),计算内生肌酐清除率(Ccr);观察肾脏病理变化及肾组织中TGF-β1和Smad7蛋白的表达。结果:与对照组相比,糖尿病肾病模型组大鼠血糖、24 h尿蛋白定量、Scr和BUN明显升高,Ccr显著降低;肾组织出现较明显的病理损伤;且肾组织TGF-β1表达显著上调,Smad7蛋白表达量明显减少。益肾胶囊治疗后,与糖尿病肾病模型组相比,24 h尿蛋白定量、Scr和BUN明显下降,Ccr显著上升;肾组织病理损伤程度减轻;此外,肾组织TGF-β1表达显著下调,Smad7蛋白表达量明显增加。结论:益肾胶囊可能通过调节TGF-β1/Smad的表达,从而对糖尿病肾病发挥肾脏保护作用。Objective：To investigate Yishen Capsule on the expression of TGF-β1and Smad7 in the kidney of the Rats with Diabetic Nephropathy.Methods：32 Wistar male rats were randomly divided into four groups：the normal control group,diabetic nephropathic model group,yishen capsule treated group and losartan treated group（eight rats in each group）,Rats in yishen capsule treated group and in losartan treated group were gived yishen capsule（625 mg · kg-1 · d-1） and losartan potassium（20 mg · kg-1 · d-1） by intragastric administration respectively for eight weeks.Blood glucose,urinary protein/24h,blood serum creatinine,blood urea nitrogen and endogenous creatinine clearance rate were measured before and after the research,the expressions of TGF-β1 and Smad7 in renal tissue were researched by immunohistochemisty.Results：Compared with the normal control group,there were significant increased blood glucose,urinary protein/24 h,Scr,BUN and decreased Ccr in the diabetic nephropathic model group.Moreover,the up-regulated expressions of TGF-β1 and the decreased expressions of Smad7 were observed in renal tissue of diabetic nephropathic model group.Furthermore,Yishen capsule could restore the up-regulatsd expressions of TGF-β1 and the decreased expressions of Smad7.Conclusion：In this paper,Yishen capsule could protect the kidney with diabetic nephropathy,which may be related to restore the expressions of TGF-β1 and Smad7.

关 键 词：益肾胶囊 糖尿病肾病 TGF-β1 SMAD7 

分 类 号：R587.2[医药卫生—内分泌] R692[医药卫生—内科学]