老年创伤患者外周血髓源抑制细胞的变化及其意义  

Variation and significance of CD14^-/CD11b^+/CD33^+myeloid derived suppressor cells in elderly patients with multiple injuries

作　　者：薛晨晨[1] 何方军 赵建立[1] 尹燕华[3] 白祥军[1] 李占飞[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院创伤外科,湖北武汉430030 [2]宜昌市夷陵区人民医院急诊科,湖北443100 [3]华中科技大学同济医学院生物化学与分子生物学系,湖北武汉430030

出　　处：《创伤外科杂志》2014年第3期215-218,共4页Journal of Traumatic Surgery

基　　金：武汉市科技计划(201161038339);国家十二五支撑计划(2012BAI11B00);湖北省医学领军人才培养工程专项经费资助

摘　　要：目的探讨老年创伤患者外周血中髓源抑制细胞(MDSCs)比例变化及其临床意义。方法收集26例青年创伤患者(18~30岁)和21例老年创伤患者(>60岁),以CD14-/CD11b+/CD33+作为MDSCs标志物,流式细胞术(FCM)检测MDSCs比例,酶联免疫吸附试验(ELISA)检测血清白介素-10、转化生长因子-β和C反应蛋白水平,分析MDSCs比例的变化情况及其临床意义。结果伤后1d,老年组和青年组创伤患者外周血MDSCs比例无显著差异(P>0.05)。伤后3d和7d,老年组创伤患者外周血MDSCs比例均高于青年组,差异有显著意义(P<0.01)。MDSCs比例和血清转化生长因子-β、白介素-10和C反应蛋白水平均有相关性。结论老年创伤患者外周血MDSCs比例较青年组显著增高,可能是老年创伤患者更容易发生免疫抑制的重要机制。Objective To explore the variation and significance of CD 14 -/CD11b＋/CD33 ＋myeloid de-rived suppressor cells （ MDSCs ） in elderly patients with multiple injuries . Methods Totally 26 young patients （ age 18-30 years ） and 21 elderly patients （ age 〉60 years ） with multiple injury were enrolled in this study . CD14 -/CD11b＋/CD33 ＋MDSCs in peripheral blood were determined by flow cytometry （ FCM） at 1,3 and 7days after trauma.Serum transforming growth factor-β（TGF-β）,interleukin-10 and C-reactive protein were determined by ELISA.Results The percentages of CD14 -/CD11b＋/CD33 ＋MDSCs in peripheral blood of elderly patients at 3 and 7days after trauma were significantly higher than those of young patients （P0.05）.The percentages of CD14 -/CD11b＋/CD33 ＋MDSCs in peripheral blood of elderly patients with multiple injury were closely correlated with serum TGF -β,interleukin-10 and C-reactive protein level （P 〈0.05）.Conclusion The percentages of CD14 -/CD11b＋/CD33 ＋MDSCs in peripheral blood were significantly increased in elderly patients with multiple injuries .Our results indicated that accumulation of CD 14 -/CD11b＋/CD33 ＋MDSCs may be an important mechanism of aging-related immune dysfunction in elderly patients with multiple injuries .

关 键 词：创伤 细胞 感染 免疫 

分 类 号：R392.3[医药卫生—免疫学]