氟中毒对大鼠内源性胱硫醚-β-合成酶及硫化氢水平的影响  

Effects of fluorosis on levels of cystathioulne beta-synthase and hydrogen sulfide in rats

作　　者：潘际刚[1,2] 楼迪栋[1] 刘艳洁[1] 魏娜[1] 刘咏妍[3] 官志忠[1,3] 

机构地区：[1]贵阳医学院病理学教研室,550004 [2]贵阳医学院生理学教研室,550004 [3]贵阳医学院分子生物学重点实验室,550004

出　　处：《中华地方病学杂志》2014年第3期268-271,共4页Chinese Journal of Endemiology

基　　金：国家自然科学基金（81160335）;科技部国际合作项目（2013BA105803）;贵州省国际科技合作计划（黔科合外G字[2011]7014号）;贵州省科技厅贵阳医学院联合基金（黔科合LG字[2012]018号、黔科合计z字[2012]4010）;贵州省科技厅科学技术基金（黔科合J字[2010]2267号）;贵阳医学院博士科研基金（K2009-8）

摘　　要：目的观察慢性氟中毒对大鼠内源性胱硫醚-β-合成酶（CBS）及硫化氢（H2S）水平的影响。方法48只SD大鼠（体质量105-180g）按体质量采用随机数字表法分为3组，每组16只大鼠（雌雄各半），其中对照组、低氟组、高氟组大鼠饲料氟含量分别为9．80、15．40、23．80mg／kg。染氟6个月后，氟离子选择电极法检测大鼠尿氟及骨氟；亚甲基蓝法检测大鼠血清及脑组织H2s含量、CBS活性；蛋白质印迹法（Westernblot）检测大鼠脑组织CBS蛋白表达。结果染氟6个月后，与对照组比较，低氟组和高氟组大鼠均有氟斑牙出现；大鼠尿氟、骨氟组间比较差异有统计学意义（，值分别为65．16、67．93，P均〈0．05）。其中尿氟和骨氟在低氟组[（5．25±0．45）mg／L，（1196．54±72．78）mg／kg]和高氟组[（13．17±0．98）mg／L，（2656．61±170．12）mg/kg]的水平均高于对照组[（3．64±0．20）mg／L，（870．71±71．51）mg／kg，P均〈0．05]，且呈剂量依赖性增加（P均〈0．01）。大鼠血清、脑组织中H2s含量组间比较差异有统计学意义（F值分别为4．83、1456．13，P均〈0．05）。其中高氟组大鼠血清H2S[（17．64±2.38）μmol／L]高于对照组[（10．29±0．74）μmol／L，P〈0．01]；低氟组和高氟组大鼠脑组织H2S[（364．74±2．06）、（513．43±4．18）μmol／L]均高于对照组[（314．94±0．72）μmol／L，P均〈0，01]，且呈剂量依赖性增加（P〈0．01）。大鼠脑组织中CBS活性组间比较差异有统计学意义（F=760．63，P〈0．01）。其中低氟组和高氟组大鼠脑组织CBS活性[（438．90±2．83）、（529．83±2．37）mmol·kg-1·min-1]低于对照组[（596．33±2．75）mmol·kg-1·min-1，P均〈0．01]。高氟组大鼠脑组织CBS蛋白表达（1．49±0．08）高于对照组（1．19±0．06，P〈0．05）。结论慢性氟中毒能够影响大鼠内源性CBS及H2S水平，除CBS活性外，�Objective To observe the effects of fluorosis on the levels of endogenous cystathionine beta- synthase （CBS） and hydrogen sulfide （H2S） in rats. Methods According to body weight, forty-eight Sprague- Dawley rats （body weight 105 - 180 g） were divided into three groups by a random number table （16 rats in each group, half male）. Fluorine contents of the feed in control group, low-fluoride group and high-fluoride group were 9.80, 15.40 and 23.80 mg/kg. After 6 months of fluorine exposure, the fluorine contents of urine and bone were determined by the method of fluorine ion-selective electrode ; H2S levels in serum and brain and the activity of CBS in brain were detected by methylene blue; and protein expression of CBS was detected by Western blotting. Results Compared with control group, dental fluorosis was found in rats of low-fluoride and high-fluoride groups. The differences of fluorine contents of urine and femur were statistically significant between groups （F = 65.16, 67.93, all P 〈 0.05）. The urinary and femoral fluorine in low-fluoride groups[（5.25 ± 0.45）mg/L, （1 196.54 ± 72.78）mg/kg] and high-fluoride groups[（13.17±0.98）mg/L, （2 656.61 ± 170.12）mg/kg] were higher than thoseof control groups [（3.64 ± 0.20）mg/L, （870.71 ± 71.51）mg/kg, all P〈 0.05], and the increases were in a dose-dependent fashion（all P 〈 0.01 ）. The differences of H2S contents in serum and brain were statistically significant（F = 4.83, 1 456.13, all P 〈 0.05）. The H2S content in serum was higher in high-fluoride group [ （17.64 ± 2.38）μmol/L] than that of the control group[ （10.29 ± 0.74）μmol/L, P 〈 0.01 ]. The H2S contents in brain were higher in the low-fluoride [ （364.74 ± 2,06）μmol/L] and high-fluoride groups [ （513.43±4.18 ） μmol/L] than those of the control group[ （314.94 ±0.72）μmol/L, all P 〈 0.01 ], and the increase was in a dose-dependent fashion （P 〈 0.01 ）. The difference of CBS activity was statistically significant betwee

关 键 词：氟中毒  牙 胱硫醚-Β-合成酶 硫化氢 大鼠 

分 类 号：R595[医药卫生—内科学]