重庆市燃煤型地方性氟中毒人群降钙素受体基因多态性与环境因素的交互作用  

Interaction of calcitonin receptor gene polymorphisms and environmental factors in the population lived in coal-burning.borne endemic fluorosis areas in Chongqing Jiang

作　　者：蒋苗[1] 牟李红[1] 王应雄[1] 晏维[2] 焦永卓[1] 

机构地区：[1]重庆医科大学公共卫生学院流行病学教研室,400016 [2]重庆市疾病预防控制中心地方病防治所

出　　处：《中华地方病学杂志》2014年第3期275-279,共5页Chinese Journal of Endemiology

摘　　要：目的探讨降钙素受体（CTR）基因多态性与环境因素在重庆市燃煤型地方性氟中毒（地氟病）人群中的交互作用。方法采用病例对照研究1：1配比设计．选取重庆市巫山县笃坪乡和奉节县兴隆镇2个燃煤型地氟病病区人群作为观察对象，其中病例组121例、内对照组130例。利用聚合酶链反应一限制性片段长度多态性（PCR-RFLP）法检测CTR基因多态性。应用条件Logistic回归模型分析重庆市燃煤型氟中毒发生的环境及遗传因素，根据交互作用指标判断基因与环境的交互作用。结果病例组中CC基因型分布低于内对照组[60．33％（73／121）比74．62％（97／130）]，而TT基因型分布明显高于内对照组[9．09％（11／21）比3．85％（5／130）]；且两组间三种基因型分布比较差异有统计学意义[χ2=6．57，P=0．037〈0．05；95％可信区间（CI）为0．029—0．036]。两组间等位基因频率分布比较差异有统计学意义[χ2=7．67，P=0．006〈0．01；比值比（OR值）=0．53，95％CI为0．338～0．834]。经Logistic回归分析，尿氟≥1mg／L为氟中毒的危险因素（OR=1．814，P：0．041〈0．05）；尿氟≥1mg／L及CTR基因的CT＋TT基因型是该人群氟中毒的促进因素，两者存在交互作用[OR=5．530，交互作用系数（γ）=2．457]。结论CTR基因C／T多态性与环境化学元素中氟含量（尿氟≥1mg／L）在重庆市燃煤型地氟病人群中存在一定的交互作用，其氟中毒现象的发生是遗传及环境因素共同作用的结果。Objective To explore the interaction of calcitonin receptor （CTR） gene polymorphisms and environmental factors in the population lived in coal-burning-borne endemic fluorosis areas in Chongqing. Methods A 1 ： 1 case-control study was carried out and Duping Township of Wushan County and Xinglong Township of Fengjie County of Chongqing were chosen as dae endemic fluorosis areas. The observation subjects were divided into case group 121 cases and internal control group 130 cases. The Alu I polymorphism in the CTR gene was genotyped using the PCR-RFLP procedure. Logistic regression model was used to analyze the environment and genetic factors, and the interaction between genes and environment was.determined according to interaction indicators. Results The rate of CC genotype in case group was lower than that of the control group [60.33% （73/121） vs. 74.62% （97/130） ], while theTY genotype was higher than that of the control group[9.09% （11/21） vs. 3.85%（5/130）]. Significant differences in Alu I genotypes were observed between groups （χ2 = 6.57, P = 0.037 〈 0.05; 95%CI： 0.029 - 0.036）. Allele frequencies of CTR genotypes differed significantly between groups（χ2= 7.67, P = 0.006 〈 0.01; OR = 0.53,95%CI： 0.338 - 0.834）. Urinary fluoride level（≥1 mg/L） was demonstrated to be a risk factor of fluorosis（OR = 1.814,P = 0.041 〈 0.05）. There was a positive interaction（OR = 5.530,γ= 2.457） between CT ＋TT genotypes in CTR and the fluorosis environment of the people （urinary fluoride level ≥ 1 mg/L）.Conclusions There is a certain type of interaction between CTR gene C/T polymorphism and environmental fluorine content （urinary fluoride ≥ 1 mg/L） in Chongqing population lived in coal-burning-borne fluorosis areas, and the onset of fluorosis is the result of interaction between heredity and environment.

关 键 词：氟中毒 多态性 降钙素受体 基因 交互作用 

分 类 号：R599[医药卫生—内科学]