布地奈德对变应性鼻炎大鼠鼻腔灌洗液中嗜酸粒细胞凋亡的影响  

Effects of budesonide on eosinophil apoptosis in nasal lavage fluid of allergic rhinitis rats

作　　者：庄晓艳 李钦[2] 陈彦林[2] 马焱燚[2] 

机构地区：[1]潍坊医学院,山东潍坊261042 [2]临沂市人民医院耳鼻咽喉科,山东临沂276003

出　　处：《山东大学耳鼻喉眼学报》2014年第2期68-71,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

摘　　要：目的建立大鼠变应性鼻炎（allergic rhinitis，AR）模型，观察布地奈德对AR大鼠鼻腔灌洗液中嗜酸粒细胞（eosinophil，EOS）凋亡的影响。方法采用卵清蛋白、氢氧化铝建立大鼠AR模型，分为AR组（B组）、布地奈德组（C组），每组10只大鼠，分别于模型建立后第31-38天，每只每日每侧鼻腔滴入磷酸盐缓冲液50汕、布地奈德50μL（1．28μg/μL），另设对照组（A组）大鼠10只。第39天取各组大鼠鼻腔灌洗液测定EOS百分比，流式细胞术检测EOS凋亡率。结栗C组大鼠鼻腔灌洗液中EOs为（5．10±0．51）％，明显低于B组的（29．37±4．33）％（P〈O．01）；C组鼻腔灌洗液中EOS凋亡率为（18．48±2．84）％，明显高于B组的（3．36±0．50）％（P〈0．01）；且B组、C组大鼠鼻腔灌洗液中EOS百分比与其凋亡率呈显著负相关（r=-0．892，P〈0．05）。结论布地奈德可明显减少AR大鼠鼻腔内EOS浸润，并能显著增加EOS凋亡，进而减轻AR的炎症反应。从而达到治疗AR的目的。Objective To observe the effect of budesonide on the eosinophil（EOS） apoptosis from nasal lavage fluid in allergic rhinitis （AR） rats. Methods AR model was established by sensitizer of ovalbumin and aluminum hydroxide. Rats were divided into the control group （ group A, n = 10）, the allergic rhinitis group （ group B, n = 10） and the budesonide group （group C, n = 10）. In days 31 to 38 after the establishment of AR, each rat of the group B was ad ministrated by instillation of 50 μL（ 1.28 μg/μL） budesonide into each of the nasal cavities one time per day, while each rat of the group C was administrated by instillation of 50 μL phosphate buffer in the same way. In days 39, the na sal lavage fluid was obtained to measure the percentage of the apoptotic eosinophils and eosinophils with flowcytometry. Results The percentage of EOS in nasal lavage fluid of the group C was 5.10± 0.51 which was evidently lower than that of the group B （29.37 ±4.33, P 〈0.01 ）. The eosinophil apoptosis rate in group C was 18.48 ±2.84 which was evident ly higher than in group B （3.36 ± 0.50, P 〈 0.01 ）. Furthermore significantly negative correlation was observed between the percentage of EOS and apoptosis rate in the nasal lavage fluid （ r = - 0. 892, P 〈 0.05 ）. Conclusion Budesonide can significantly reduce the eosinophil infiltration and increase eosinophil apoptosis so as to treat allergic rhinitis.

关 键 词：模型 动物 变应性鼻炎 嗜酸粒细胞 凋亡 鼻腔灌洗液 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]