药物洗脱球囊抑制下肢动脉狭窄性病变的实验研究  

The inhibition effect of novel drug-eluting balloon on obstructive peripheral arterial disease of lower extremity: an experimental study in rabbit models

作　　者：张文俐[1] 杜润[1] 朱政斌[1] 朱劲舟[1] 应晨[1] 刘慧竹[1] 张瑞岩[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院心内科,200025

出　　处：《介入放射学杂志》2014年第5期423-426,共4页Journal of Interventional Radiology

摘　　要：目的探讨新型药物洗脱球囊（DEB）在下肢动脉狭窄性病变（PAD）中可能具有的抑制管腔丢失、减少内膜增生作用。方法以雄性新西兰纯种兔（体重2．5～3．0kg）为实验对象，于兔腹主动脉近心端及远心端各植入Mustang裸支架1枚，模拟PAD模型，并分别用裸球囊（胛A组）及药物洗脱球囊（DEB组）在血管支架段行后扩张，术后28d处死动物，收集腹主动脉支架标本，塑料包埋并行Masson染色，比较各组内膜增生程度，同时行DEB药物（紫杉醇）靶点B．微管蛋白免疫组化检测。结果形态学上，与PrrA组比较，DEB组内膜增生及管腔狭窄率明显下降。同时B．微管蛋白免疫组化检测显示．与门A组相比，DEB组血管内膜p-微管蛋白表达量显著增加。结论动物实验初步显示，DEB具有潜在的有效地抑制管腔丢失、减少内膜增生作用，初步证实该新型DEB的优良性能，提示该新型DEB远期应用于临床治疗PAD的潜在可能性。Objective To investigate the possible inhibition effect of a domestic novel drug-eluting balloon（DEB）, such as controlling the vascular stenosis and reducing the intimal hyperplasia, on obstructive peripheral arterial disease （PAD） of lower extremity. Methods Male New Zealand rabbits （weighted 2.5 - 3.0 kg） were used for this study. Mustang bare metal stents （BMS） were separately implanted at both the proximal and distal site of abdominal aorta to establish the PAD models in rabbits. Bare balloon （PTA group） or drug-eluting balloon （DEB group） was separately used to dilate the stent segment of the aorta. The animals were sacrificed 28 days after the treatment. The aorta stent specimens were collected and sent for pathologic examination. Using Masson staining method the degree of intimal hyperplasia was estimated and the expression level of paclitaxel- targeted β- tubulin was determined with immunohistochemical method. The results were compared between the two groups. Results Morphologically, both the degree of intimal hyperplasia and the vascular stenosis rate in DEB group were significantly lower than those in PTA group. Meanwhile, immunohistochemical examination of paclitaxel-targeted 13-tubulin indicated that its expression level in DEB group was obviously higher than that in PTA group. Conclusion This experimental study indicates that the domestic novel DEB has potential inhibition effect in respect of controlling the loss of lumen as well as reducing the intimal hyperplasia. This novel DEB carries excellent property, suggesting the possibility to use DEB in clinical treatment of PAD.（J Intervent Radiol, 2014, 23： 423-426）

关 键 词：下肢动脉狭窄性病变 药物洗脱球囊 紫杉醇 内膜增生 

分 类 号：R543.5[医药卫生—心血管疾病]