血清8-羟基脱氧鸟苷与糖尿病性视网膜病变相关性  

The relationship of serum 8-hydroxydeoxyguanosin to diabetic retinopathy

作　　者：柯毅[1] 黎宗汉[1] 叶丽[1] 李瑞庄[1] 周美英[1] 方林彬[1] 

机构地区：[1]广东医学院附属医院眼科,广东湛江524001

出　　处：《中国实用眼科杂志》2014年第5期638-640,共3页Chinese Journal of Practical Ophthalmology

摘　　要：目的探讨2型糖尿病（T2DM）患者血清8-羟基脱氧鸟苷酸（8-OHdG）和糖尿病性视网膜病变的关系。方法对2012年12月至2013年3月在广东医学院附属医院眼科住院的49例T2DM患者根据视网膜损害的不同，分成无糖尿病性视网膜病变组（DM组，12例）、非增殖性糖尿病性视网膜病变组（NPDR组，19例）和增殖性糖尿病性视网膜病变组（PDR组，18例），采用酶联免疫吸附法（ELISA）测定其血清8-OHdG水平，并与健康体检者（NC组，22人）进行比较。结果T2DM组和对照组8-0HdG水平不满足正态分布及方差齐性，用WillcoxonW秩和检验进行两组比较，差异有统计学意义（Z=-6．70，P〈0．01），T2DM组8-0HdG水平（平均秩和为47．00）高于对照组（平均秩和为11．50）；Kruskal—Wallis秩和检验法比较T2DM3个亚组间血清8-0HdG水平差异有统计学意义（X^2=59．50，P=0．00），PDR组8-OHdG水平最高（平均秩和为38．08），NPDR组次之（平均秩和为22．68），DM组最低（平均秩和为9．04）。Spearman相关分析表明T2DM患者血清8-OHdG与糖尿病性视网膜病变程度呈正相关（r=0．94，P=0．00）。结论T2DM患者的血清8-OHdG水平的增高与糖尿病性视网膜病变有关，提示血清8-OHdG水平增高是T2DM患者合并糖尿病性视网膜病变的危险因素之一。Objective To investigate the relationship of the levels of serum 8-hydroxydeoxyguano-sin （8-OHdG） to diabetic retinopathy （DR） in subjects with type 2 diabetes （T2DM）. Methods The patients were classified into groups according to retinopathy （no signs of diabetic retinopathy [DM], nonproliferative diabetic retinopathy [NPDR], and proliferative diabetic retinopathy [PDR]）. The concen-tration of serum 8-OHdG were measured with enzyme-linked immunosorbent assay （ELISA） in all 49 patients with diabetes mellitus and 22 normal control subjects. The correlation between 8-OHdG and di-abetic retinopathy were analyzed in all patients with T2DM. Results The concentration of serum 8-OHdG in patients with T2DM were significantly higher than those in the control subjects （Willcoxon W Test, Z=-6.70, P=0.00; Mean Rank of T2DM group and normal control subjects are 47.00 and 11.50 respectively）. There was statistical difference in the levels of serum 8-OHdG in the PDR, NP-DR, and DM groups （Kruskal Wallis Test, X^2=59.50, P =0.00）. The levels of serum 8-OHdG was much higher in the patients with PDR than that in patients with diabetes without retinopathy and with NPDR, The concentration of serum 8-OHdG was higher in the patients with NPDR than that in pa- tients with diabetes without retinopathy. The degree of 8-OHdG was correlated with the degree of DR （Spearman correlation coefficients=0.94, P =0.00）. Conclusions The elevated serum 8-OHdG may be one of probable risk factors in the diabetic retinopathy in the patients with T2DM.

关 键 词：2型糖尿病 8-羟基脱氧鸟苷酸 糖尿病性视网膜病变 

分 类 号：R587.2[医药卫生—内分泌]