金叶榆ISSR识别卡构建及8份榆属植物材料的亲缘关系分析  

Construction of ISSR identification card of Ulmus pumila‘Jinye’and analysis of genetic diversity of 8 Ulmus materials

作　　者：代波[1] 杜金友[2] 杨俊明[1] 黄印冉 张均营[3] 

机构地区：[1]河北科技师范学院园艺科技学院 [2]河北科技师范学院生命科技学院 [3]河北省林业科学研究院

出　　处：《北京林业大学学报》2014年第3期100-103,共4页Journal of Beijing Forestry University

基　　金："十一五"国家科技支撑计划项目(2009BADB0105)

摘　　要：采用ISSR分子标记技术对8份(品种、类型)榆属植物材料进行遗传多样性分析,从78个引物中筛选出12个具有扩增多态性的引物用于ISSR-PCR扩增,共扩增出74条带,其中多态性条带63条,占总扩增条带的85%。用NTSYSpc2.10e软件对ISSR扩增结果进行分析,结果表明,供试材料具有较丰富的遗传多样性。利用扩增条带存在赋值1,否则赋值0的数据分析方法建立金叶榆ISSR识别卡;利用UPGMA法进行聚类分析,将8份(品种、类型)植物材料按亲缘关系远近分为4类。Inter-simple sequence repeat （ISSR） marker was used to analyze genetic diversity of 8 improved breeds of Ulmus spp. Twelve primers screened out from 78 primers were used for ISSR-PCR. A total of 74 bands were generated, of which 63 bands appeared to be polymorphic, occupied 85% of total. The amplification results of ISSR were analyzed by NTSYSpc 2.10e software, and indicated that there actually existed high genetic diversity at molecular level among the 8 Ulmus materials. The method of amplified band assignment in 1, otherwise 0 was used to establish a ISSR identification card. These 8 improved breeds of Ulmus materials were divided into four groups with ISSR marker by UPGMA method.

关 键 词：榆树 遗传多样性 ISSR 生物技术 

分 类 号：S718.46[农业科学—林学] S718.49