大叶蚁塔组织培养技术  

Tissue Culture of Gunnera manlcata L.

作　　者：陈锁良 吕秀立[2] 崔心红[2] 王铖[2] 张群[2] 朱义[2] 陆亮[2] 

机构地区：[1]江苏生太环境建设集团有限公司,丹阳212300 [2]上海市园林科学研究所,上海200232

出　　处：《植物研究》2014年第3期328-332,共5页Bulletin of Botanical Research

基　　金：国家林业局公益性行业科研专项:城市湿地生态系统健康评价与恢复技术研究(201104088)项目资助

摘　　要：以大叶蚁塔茎尖作为外植体诱导出无菌苗,再以无菌苗的嫩叶诱导不定芽发生,研究了其离体培养和不定芽再生的过程,成功建立了低成本的快速繁殖技术体系。结果表明:(1)无菌苗增殖培养基为MS+BA 1.0mg·L-1+NAA 0.1 mg·L-1,培养30 d,增殖率稳定为3.80;(2)叶片可直接诱导再生出不定芽,其最佳培养基为MS+ZT 1.0 mg·L-1+NAA 0.1 mg·L-1,诱导率达95%,分化幼苗众多;(3)生根培养基为1/2MS+NAA1 mg·L-1+蔗糖20 g·L-1时,生根率达95%。Using the apical buds of Gunnera manlcata as explants to induce the aseptic the new leaves of aseptic seedling to induce the adventitious buds, apical buds of G. manlcata were studied in this paper, and the seedlings and then using in vitro culture and regeneration process of quick propagation system in low cost was successfully established. The result showed： （ 1 ） Multiplication stage ： MS ＋ BA 1.0 mg · L - 1 ＋ NAA 0. 1 mg · L- -, cultured for 30 d, the stable multiplication rate was 3.80; （2） Induction stage： MS ＋ ZT 1.0 mg · L-1 ＋ NAA 0.1 mg · L-1, which was proved to be superior to the others to be used to induce apical buds directly from the leaf explants, the induction rate reached 95% ; （3） Rooting stage： 1/2MS ＋ NAA 1 mg · L-1 ＋ sugar 20 g · L-1 , the rooting rate reached 95%.

关 键 词：大叶蚁塔 离体培养 快速繁殖 

分 类 号：Q949.762.6[生物学—植物学]