木豆毛状根生长特性及相关生理生化指标研究  

Characteristics and Biochemical Indexes of Cajanus cajan Hairy Root

作　　者：李敏 曲丹[2] 于海娣[2] 王慧梅[2] 

机构地区：[1]中国林业出版社,北京100009 [2]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2014年第3期339-342,共4页Bulletin of Botanical Research

摘　　要：木豆毛状根液体培养过程中生长特性、碳源消耗情况及次生代谢产物合成规律,为木豆毛状根规模培养提供基础数据。结果表明:木豆毛状根的生长代谢周期为25 d,其生长经历了延迟期、快速生长期以及生长平缓期;在木豆毛状根的生长周期内,木豆毛状根的生长速度和碳源的消耗存在明显的正比关系,生长速度越快,碳源消耗越多。随着生长速度趋于平缓,碳源消耗也趋于平缓。芹菜素与染料木素的含量都是随着培养时间的延长而不断增加的,到生长平缓期趋于平稳。In this paper, the characteristics, biosynthesis pattern of secondary metabolites and carbon consumption in the liquid culture of Cajanus cajan hairy root were studied. Results showed that the metabolic cycle of C. cajan hairy root was 25 days including the experienced delayed phase, growing fast phase and steady phase. In C. cajan hairy root growth cycle, its growth rate and consumption of carbon were highly related, the faster growth rate, the more carbon consumption. With leveled off. The contents of apigenin and genistein in became steady in the moderate growth period. the growth rate becoming steady, carbon consumption the hairy root increased with the time increasing but became steady in the moderate growth period.

关 键 词：木豆毛状根 生长周期 碳源消耗 次生代谢产物 

分 类 号：Q949.751.9[生物学—植物学]