山新杨组培苗生根移栽方法  

Rooting and Transplanting Method of Populus davidiana × P.bolleana Tissue Culture Seedlings

作　　者：王娜[1] 窦恺[2] 王志英[2] 张荣沭[3] 王玉成[1] 刘志华[2] 

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,哈尔滨150040 [2]东北林业大学林学院,哈尔滨150040 [3]东北林业大学园林学院,哈尔滨150040

出　　处：《植物研究》2014年第3期380-385,共6页Bulletin of Botanical Research

基　　金：黑龙江省博士后科研启动金(LBH-Q10156);黑龙江省青年科学基金项目(QC2011C003);国家自然科学基金面上项目(31170601);哈尔滨市科技创新人才研究专项资金项目(2011RFQXN051)

摘　　要：从炼苗，保湿，防污染等方面，研究出一种新型高效的山新杨组培苗生根移栽方法。首先，获取山新杨组培苗，将其用液体生根培养基在大试管中生根12～15d；再放入无菌自来水中水培炼苗2d；之后将幼苗移栽入土，上扣透明塑料水杯保湿，15d后移去水杯；带土移栽到大田中。此法可使组培苗移栽成活率提高至98％以上，且本法经济简便，规模小，效率高，也适用于其它木本植株，便于推广。A new typical and high eificient rooting and transplanting method ot Poputus davidana x r. Ootteana was set up from hardening off seedlings, moisturizing and pollution prevention in the study. Firstly, put the tissue culture P. davidiana x P. bolleana seedlings in large test tubes with liquid medium for rooting for 12 - 15 d. Secondly, refine hydroponic seedlings in the aseptic water for 2 d. Then, transplant the seedlings into soil and cover with plastic cups to moisturizing, which will be removed after 15 d. At last, the 17 d old seedlings with soil were transplanted into the field. Using the above method, the survival rate of transplanting seedlings can increase to more than 98%. The advantage of the method is not only economic, simple, small-scaled and high efficient but also suitable for many other woody plants, so it is easy to generalize.

关 键 词：山新杨 组织培养 生根 移栽 成活率 

分 类 号：S792.11[农业科学—林木遗传育种]