一种新型靶向微泡-干细胞复合体的制备  

Preparation of a novel targeted microbubbles-stem cell complexes

作　　者：岳媛媛[1,2] 王志刚[1,2] 张萍[1,2] 柯青兰[1,2] 何子朋[1,2] 罗田田[1,2] 

机构地区：[1]重庆医科大学超声影像学研究所,重庆400010 [2]重庆医科大学附属第二医院超声科,重庆400010

出　　处：《中国医学影像技术》2014年第5期680-684,共5页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金重点项目(81130025);重庆市教委科学技术研究项目(KJ110320);重庆高校创新团队建设计划(KJTD201303)

摘　　要：目的制备一种新型靶向微泡-干细胞复合体。方法采用"生物素-亲和素"桥连法构建携抗ICAM-1抗体的靶向微泡(MBICAM-1)。用带正电荷的多聚赖氨酸(PLL)对MBICAM-1进行包覆和修饰,使靶向微泡表面带正电荷。采用静电吸附法将PLL修饰的MBICAM-1与大鼠骨髓间充质干细胞(MSC)共同孵育,制备MBICAM-1-MSC复合体;以流式细胞仪检测靶向微泡与MSC的结合率。结果经PLL修饰后,MBICAM-1微泡表面带正电荷,但微泡形态、粒径以及与损伤内皮细胞(HUVEC)的结合率与未经PLL修饰的MBICAM-1无显著差异(P>0.05)。PLL修饰的MBICAM-1与MSC结合形成MBICAM-1-MSC复合体,当MSC与MBICAM-1之间的比例为1∶40时,二者结合率达(29.45±2.88)%。结论成功制备了MBICAM-1-MSC复合体;MSC与MBICAM-1的比例为1∶40时,其结合率最大。Objective To prepare a novel targeted microbubble-stem cell complexes. Methods ICAM-1-targeted microbubbles （MB_ICAM-1） were prepared by using biotin-avidin bridging chemistry method. Subsequently, MB_ICAM-1 were coated and modified by binding positively charged polylysine （PLL） to their surface.Rat marrow mesenchymal stem cells （MSC） were mixed with PLL modified MB_ICAM-1, allowing for electrostatic interaction. The attachment rate of MB_ICAM-1 to MSCs was tested with flow cytometry. Results The surface potential of PLL modified MB_ICAM-1 was positive. There was no sig-nificant difference of the morphous, diameter and attachment rate to damaged human umbilical vein endothelial cells （HU-VEC） between PLL modified MB_ICAM-1 and premodified MB_ICAM-1 （P〉0.05）. MB_ICAM-1-MSC complexes were prepared by successfully attaching PLL modified MB_ICAM-1 to MSC. Meanwhile, the attachment rate of MSC to MB_ICAM-1 was （29.45 ± 2. 88） % at the mix ratio of 1：40. Conclusion MB_ICAM-1-MSC complexes are prepared successfully using the above method. The attachment rate of MSC to MB_ICAM-1 was maximal at the mix ratio of 1：40 （MSC/MB_ICAM-1）.

关 键 词：靶向 微泡 骨髓 间充质干细胞 复合体 

分 类 号：R445.1[医药卫生—影像医学与核医学]