In vivo assessment of hepatotoxicity,nephrotoxicity and biodistribution using 3-aminopropyltriethoxysilane-coated magnetic nanoparticles(APTS-MNPs) in ICR mice  

作　　者：Xiaowen Wang Jieying Zhang Xin Yang Zhenghai Tang Yanwen Hu Benke Chen Jintian Tang 

机构地区：[1]Institute of Neurological Disorders,Yuquan Hospital,Tsinghua University [2]Department of Biological Pharmaceuticals,Beijing University of Chinese Medicine [3]Key Laboratory of Particle & Radiation Imaging,Tsinghua University,Ministry of Education

出　　处：《Chinese Science Bulletin》2014年第16期1800-1808,共9页

基　　金：supported by the National Natural Science Foundation of China (81071885)

摘　　要：The biocompatibility and biodistribution of magnetic nanoparticles(MNPs)in vivo are essential to ensure their safely clinical application.We have studied these aspects with our 3-aminopropyltriethoxysilanecoated magnetic nanoparticles(APTS-MNPs)formulation,which can be used as magnetic induction hyperthermia media.Changes in tissue iron levels were analyzed after intraperitoneal injection of APTS-MNPs to ICR mice.Liver and kidney functions were tested.Heart,liver,spleen,lung,kidney,testis,and brain were sectioned for pathological analysis.Biodistribution of iron in various body tissues changed with time but greater fraction of the injected iron localized in the liver and spleen than in other tissues.Serum showed an increase in AST and LDH following APTS-MNPs injection.Histological analyses of selected tissues showed no obvious abnormal changes.In conclusion,APTS-MNPs did not cause continuing changes in the liver and kidney function and thus can be safely used for in vivo application.The biocompatibility and biodistribution of magnetic nanoparticles （MNPs） in vivo are essential to ensure their safely clinical application. We have studied these aspects with our 3-aminopropyltriethoxysilane-coated magnetic nanoparticles （APTS-MNPs） formulation, which can be used as magnetic induction hyperthermia media. Changes in tissue iron levels were analyzed after intraperitoneal injection of APTS-MNPs to ICR mice. Liver and kidney functions were tested. Heart, liver, spleen, lung, kidney, testis, and brain were sectioned for pathological analysis. Biodistribution of iron in various body tissues changed with time but greater fraction of the injected iron localized in the liver and spleen than in other tissues. Serum showed an increase in AST and LDH fol-lowing APTS-MNPs injection. Histological analyses of selected tissues showed no obvious abnormal changes. In conclusion, APTS-MNPs did not cause continuing changes in the liver and kidney function and thus can be safely used for in vivo application.

关 键 词：磁性纳米粒子 ICR小鼠 生物分布 肝毒性 MNP 肾毒性 乙氧基 氨丙基 

分 类 号：R692[医药卫生—泌尿科学]