Near-infrared fluorescent aptamer-templated silver nanoclusters facilely synthesized for cellular imaging applications  

作　　者：Shengliang Li Weipeng Cao Shubin Jin Chunqiu Zhang Juan Liu Guozhang Zou Feng Li Xing-Jie Liang 

机构地区：[1]Department of Neurobiology and Anatomy,Zhongshan School of Medicine,Sun Yat-sen University [2]Laboratory of Nanomedicine and Nanosafety,Division of Nanomedicine and Nanobiology,National Center for Nanoscience and Technology of China [3]CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,Chinese Academy of Sciences

出　　处：《Chinese Science Bulletin》2014年第16期1868-1872,共5页

基　　金：supported by the National Natural Science Foundation of China(81171455,81271476,31225009);the National Basic Research Program of China(2009CB930200);the‘‘Hundred Talents Program’’of Chinese Academy of Sciences(07165111ZX);the Chinese Academy of Sciences Knowledge Innovation Program;the National High Technology Research & Development Program of China(2012AA020804)

摘　　要：Fluorescent metal nanoclusters(NCs)have received extensive attention for their potential uses in bionanotechnology.Here,we develop a facile strategy to synthesize near-infrared fluorescent silver nanoclusters(Ag NCs)stabilized by MUC1 aptamer.The MUC1-Ag NCs are characterized by UV–Vis absorption spectroscopy,fluorescence spectroscopy,transmission electron microscopy,and fluorescence lifetime.These results indicated that the MUC1-Ag NCs possess bright near-infrared luminescence,high stability,and excellent biocompatibility.The cellular imaging of MUC1-Ag NCs by confocal laser microscopy demonstrated them to be promising candidates as novel fluorescent probes for biomedical application.Fluorescent metal nanoclusters （NCs） have received extensive attention for their potential uses in bionanotechnology. Here, we develop a facile strategy to synthesize near-infrared fluorescent silver nanoclusters （Ag NCs） stabilized by MUCl aptamer. The MUCl-Ag NCs are characterized by UV-Vis absorption spectroscopy, fluorescence spectroscopy, transmission electron micros-copy, and fluorescence lifetime. These results indicated that the MUCl-Ag NCs possess bright near-infrared luminescence, high stability, and excellent biocompatibil-ity. The cellular imaging of MUCl-Ag NCs by confocal laser microscopy demonstrated them to be promising can-didates as novel fluorescent probes for biomedical application.

关 键 词：生物医学应用 近红外发光 荧光光谱 纳米团簇 合成 适体 激光共聚焦显微镜 透射电子显微镜 

分 类 号：R310[医药卫生—基础医学]