XDBM生物相容性以及促进大鼠桡骨缺损修复  

THE BIOCOMPATIBILITY OF XENOGENEIC DEMINERALIZED BONE MATRIX AND ITS APPLICATION ON PROMOTING THE REPAIR RAT RADIUS DEFECTS

作　　者：甘洪全[1] 刘鑫[2] 赵济华[3] 李琪佳[4] 

机构地区：[1]河北联合大学附属医院骨科,河北省唐山市063000 [2]开滦总医院检验科 [3]河北联合大学临床医学院 [4]河北联合大学医学实验中心

出　　处：《中国煤炭工业医学杂志》2014年第5期768-771,共4页Chinese Journal of Coal Industry Medicine

基　　金：国家科技部科技支撑课题(编号2012BAE06B03);河北联合大学科学研究基金项目资助(编号:Z201339)

摘　　要：目的探讨异种脱钙骨基质(Xenogeneic Demineralized Bone Matrix,XDBM)的体内、外生物相容性以及促进骨缺损修复愈合,为临床应用提供实验依据。方法新生SD颅盖骨成骨细胞培养。取人及大鼠松质骨制备人(异种)及大鼠(同种)脱钙骨基质;观察成骨细胞在XDBM表面的黏附、增殖;行一侧大鼠骶棘肌肌袋内植入异种脱钙骨基质(实验组),对侧植入同种脱钙骨基质(对照组)。分别于术后2,4,8周取材。另取8周龄大鼠造桡骨中段5mm节段性骨缺损模型。分为异种脱钙骨组(实验组)及同种脱钙骨组(对照组)。分别于2、4、8、12周处死动物并取材。大体、组织学观察及CD34免疫组化染色计数微血管密度。结果成骨细胞在XDBM贴壁生长、增殖、分泌骨基质;XDBM植入肌袋内各时间点均未见炎症、坏死及排斥。桡骨缺损区域微血管密度增加,新骨形成最终缺损区被新骨代替。结论 XDBM具有良好的生物相容性、可降解性及良好骨传导及骨诱导作用。当植入大鼠桡骨缺损后具有促进骨再生的能力,有望成为理想的骨组织工程支架材料。Objective To explore the biocompatibility of xenogeneic demineralized bone matrix （XDBM） in vitro and vivo and the osteogenic ability in repairing the bone defect to provide experimental basis for clini- cal application. Methods The cranial- derived osteoblasts were isolated from newborn SD rats. Xenogeneic and allogenic demineralized bone matrixes were made which respectively came from a human and a rat. The adhesion and proliferation of osteoblasts on the surface of XDBM were detected. The XDBM was planted into one side of muscles sacrospinalis of rats （as the experimental group）, and planted the allogenic demineralized bone matrix into the other side （as the control group）. Specimens were collected respectively after operation 2, 4,8 weeks. The 5mm segmental radius defects in rats model was collected in thirty- two SD rats about eight- week- old which divided randomly into the XDBM group and the control group respectively. Animals in each group were put to death at 2, 4, 8, 12 week postoperatively. The general histological of the specimens were examined. CD34 immunohistochemical staining was used to determine the microvessel density （MVD）. Results Osteoblasts could grow and proliferate on XDBM and secrete bone matrix. There showed no reject reaction, inflammation or tissue necrosis in rat muscles in XDBM implantation sites. The microvessel density increased in radius defect. The newbone defect areas were replaced by new bones. Conclusions XDBM showed good biocompatibility and biodegradability, and has good conduction and induction. When the radius defect of rats is implanted, it has the ability to promote the bone regeneration, and has the respect to become an ideal an scaffold materials for bone tissue engineering.

关 键 词：异种脱钙骨基质 生物相容性 骨缺损 同种脱钙骨基质 大鼠 

分 类 号：R318.08[医药卫生—生物医学工程]