血脂对大鼠离体动脉环张力的影响  

Effects of High Fat Diet on Arterial Ring＆#39;s Tension in SD Rats

作　　者：张怡[1] 詹维伟[1] 吴永杰[2] 周吴刚[2] 陈栋睿[2] 蒋为民[3] 郑琳[3] 

机构地区：[1] 上海交通大学医学院附属瑞金医院超声科,上海200025 [2] 上海交通大学医学院附属瑞金医院高血压研究所,上海200025 [3] 上海交通大学医学院病理学教研室,上海200025

出　　处：《实验动物与比较医学》2014年第2期126-130,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的 观察高脂血症大鼠及动脉粥样硬化大鼠腹主动脉环张力的变化,方法 SD大鼠随机分为4组（T1组,n=10,高胆固醇喂养4周;T2组,n=10,高胆固醇喂养12周;C1组,n=10,正常饮食4周;C2组,n=10,正常饮食12周）,各组动物均测定血清血脂浓度及腹主动脉环对苯肾上腺素,高钾溶液及乙酰胆碱的反应,并均行病理切片,再光镜检查以评估主动脉粥样硬化的程度.结果 T1组的最大收缩张力值及最大舒张百分比显著低于C1组（P＜0.01;P=0.001）. T2组的最大收缩张力值显著高于C2组（P＜0.05）,最大舒张百分比显著低于C2组（P＜0.001）. T2组的最大收缩张力值显著高于T1（P＜0.05）.相关性分析结果显示：最大收缩张力值与甘油三酯呈显著正相关（r=0.435,P＜0.05）.最大舒张百分比与收缩压、总胆固醇及低密度脂蛋白呈显著负相关（r=-0.318,P=0.046; r=-0.449,P=0.004; r=-0.411,P=0.008）,与高密度脂蛋白呈显著正相关（r=0.324,P=0.041）.结论 高脂饮食使大鼠主动脉早期收缩与舒张能力均降低,动脉环最大舒张百分比随着总胆固醇及低密度脂蛋白水平的增高而减小.Objective To observe the changes of arterial ring＇s tension in SD rats with hypercholes- terolemia and atherosclerosis. Methods In vitro isometric tention experiment was used to measure arterial ring＇s maximum contractile tension, arterial ring＇maximum relaxation pencentage of the aorta in cholesterol-fed SD Rats （group T1, n=10, for 4 weeks; group T2, n=10, for 12 weeks） and normal control rats（group C1, n=10; group C2, n =10）. All parameters and blood biochemical markers（total cholesterol, triglycerides, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol） among groups were analyzed with ANOVE factor analysis.Correlation was analyzed with Pearson analysis. Light microscopic evaluation was used to demonstrate atherosclerotic changes in the aorta. Result The maximum contractile tension value to KC1 and phenephrine and the maximum relaxation pencentage of the aorta in group T1 were significantly lower than those of group C1 controls （P〈0.05）. The maximum relaxation pencentage of the aorta in group T2 rats were significantly lower than those of group C2 controls （P〈0.05）. The maximum contractile tension value to KCI and phenephrine in group T2 were significantly higher than those of group TI（P=0.001, P〈0.05）. However, no significances were found with the maximum relaxation percentage in group T1 and T2 （P〉0.05）. Correlation analysis shows： the maximum contractile tension value of the aorta was positively correlated with triglycerides （r=0.435, P〈0.05）. The maximum relaxation pencentage of the aorta was negatively correlated with systolic pressure, total cholesterol and low-density lipoprotein cholesterol （r=-0.318, P=0.046, r=-0.449, P=0.004; r=-0.411, P=-0.008）, also it was negatively correlated with high-density lipoprotein cholesterol（r=0.324, P=-0.041）. Light microscopy confirmed morphologic typical changes of aortic atherosclerosis in group T1 and T2. Conclution High fat diet will lower the ability ofrats＇s arterial contractio

关 键 词：高脂血症 动脉环张力 动脉粥样硬化 SD大鼠 

分 类 号：R589.2[医药卫生—内分泌] R-332[医药卫生—内科学]