1,25-二羟维生素D_3对严重烫伤小鼠应激反应的影响  

Effect of 1,25-Dihydroxyvitamin D_3 on Alleviating the Stress Response in Severely Scalded Mice

作　　者：刘曼[1] 张明谏[2] 李小兵[2] 刘光晶[2] 王玉亮[3] 张建[1] 刘宁[2] 

机构地区：[1]天津医科大学一中心临床学院,300192 [2]天津市第一中心医院整形与烧伤外科 [3]卫生部危重病急救医学重点实验室

出　　处：《天津医药》2014年第5期451-454,共4页Tianjin Medical Journal

摘　　要：目的探讨1,25-二羟维生素D3[1,25-(OH)2VitD3]对严重烫伤小鼠应激状态下胰岛素抵抗和炎症反应的影响。方法 130只小鼠按随机数字表法分为健康组10只,实验组Ⅰ、实验组Ⅱ、对照组各40只。实验组Ⅰ、实验组Ⅱ和对照组制成30%全身体表面积(TBSA)Ⅲ度烫伤模型。每隔1日晨8时,实验组Ⅰ小鼠1,25-(OH)2VitD31μg·kg-1+0.6 mL花生油灌胃;实验组Ⅱ1,25-(OH)2VitD34μg·kg-1+0.6 mL花生油灌胃;对照组0.6 mL花生油灌胃。分别于伤后1、3、7、14 d测空腹血糖(FBG)、空腹胰岛素(FIns)、血清肿瘤坏死因子(TNF)-α浓度和创面组织核因子(NF)-κB阳性率。结果 (1)实验组Ⅰ、实验组Ⅱ和对照组各时点胰岛素抵抗指数(HOMA-IR)、血清TNF-α水平和创面组织NF-κB表达阳性率均数均高于健康组水平。(2)实验组Ⅰ和实验组Ⅱ伤后同一时点HOMA-IR均低于对照组,且实验组Ⅱ低于实验组Ⅰ(P<0.05);同一组内不同时点伤后第3天HOMA-IR均数最高,以后逐渐降低(P<0.05)。(3)实验组Ⅰ和实验组Ⅱ伤后同一时点血清TNF-α水平、创面组织NF-κB表达阳性率均低于对照组,且实验组Ⅱ低于实验组Ⅰ(P<0.05);同一组内不同时点血清TNF-α水平、创面组织NF-κB表达阳性率均逐渐降低(P<0.05)。结论 1,25-(OH)2VitD3能够减轻严重烫伤小鼠应激状态下胰岛素抵抗作用和炎症反应。Objective To explore the effect of 1,25-dihydroxyvitamin D3 [1,25-（OH）2VitD3] on insulin resistance and inflammatory response in mice with severe burns under stress. Methods One hundred and thirty mice were randomly divided into healthy group （n=10）, experimental groupⅠ（n=40）, experimental groupⅡ（n=40） and control group （n=40）. The model mice were scalded to 30%of total body surface area （TBSA） burnt （Ⅲ＆#176;） in experimental groupⅠ, the experi-mental groupⅡand control group. Mice in experimental groupⅠwere given by gavaging 1,25-（OH）2VitD3 （1μg＆#183;kg-1） with 0.6 mL peanut oil at 8 am for every other day. At the same time and by the same way, mice in experimental groupⅡreceived 1,25-（OH）2VitD3 （4μg＆#183;kg-1） with 0.6 mL peanut oil, and mice in control group received only 0.6 mL peanut oil. The serum levels of fasting blood glucose （FBG）, fasting insulin （FIns）, TNF-αconcentration, and the NF-κB positive rate in wound tis-sues were determined respectively on day1, day3, day7 and day14 after burn. Results （1） The values of insulin resistance index （HOMA-IR）, serum TNF-αand the NF-κB positive rate in wound tissues were higher at different time points in exper-imental groupⅠ, experimental groupⅡand control group than those in healthy group. （2） The levels of HOMA-IR at the same time points were significantly lower in experimental groupⅠand experimental groupⅡthan those in control group, and the value was significantly lower in experimental groupⅡthan that of experimental groupⅠ（P〈0.05）. The level of HOMA-IR was the highest at day 3 and then gradually decreased at the different time points in the same group （P〈0.05）. （3） The se-rum levels of TNF-αand NF-κB positive rate of wound tissues at the same time points were significantly lower in experimen-tal group Ⅰand experimental group Ⅱ than those in control group, and the value was significantly lower in experimental groupⅡthan that of experime

关 键 词：骨化三醇 烧伤 胰岛素抵抗 肿瘤坏死因子-Α 小鼠 1 25-二羟维生素D3 1 25-dihydroxyvi-tamin D3 

分 类 号：R644[医药卫生—外科学]