PLCε基因敲除小鼠移植性肝癌模型的建立与分析  

Establishment and analysis of a PLCε knockout mouse model of transplanted liver cancer

作　　者：李晓娟[1] 靳雪源[2] 白冰珂[1] 李蓓[1] 侯俊[1] 李瑞生[1] 

机构地区：[1]解放军第302医院实验技术研究保障中心,北京100039 [2]解放军第302医院国际肝病诊疗中心,北京100039

出　　处：《中国比较医学杂志》2014年第4期5-7,I0002,共4页Chinese Journal of Comparative Medicine

摘　　要：目的利用PLCε基因敲除小鼠建立移植性肝癌动物模型,以探讨PLCε基因在肝肿瘤生长发育过程中的作用。方法选取PLCε+/+小鼠15只为对照组,选取PLCε+/+(野生型)和PLCε-/-(敲基因型)小鼠各15只为模型组I和II,沿腹中线开腹后将H22细胞接种到模型组小鼠肝脏实质内,对照组注射生理盐水。于注射后第15天行剖腹探查,观察各组成瘤率及肿瘤体积,并进行肿瘤病理学分析。结果各组小鼠的存活率均为100%。模型组I肿瘤移植成功率为100%,肿瘤体积平均为(65.21±5.25)mm3。模型组II的肿瘤移植成功率为53.3%,肿瘤体积平均为(23.46±3.47)mm3。模型组I的肿瘤平均体积明显大于模型组II(P<0.05)。模型I组和II组病理检查均证实为原位肝细胞瘤。结论成功建立了PLCε敲基因小鼠移植性肝癌动物模型,验证了PLCε敲基因小鼠具有抑制肝肿瘤生长的特性。Objective To investigate the PLCεgene function during growth and development of liver tumor using PLCεknockout mouse models of transplanted liver carcinoma .Methods Fifteen PLCε＋/＋mice were selected randomly as control group,and 15 PLCε＋/＋（wild type）and 15 PLC-/-（knockout genotype）as the model groups I and II.H22 liver carcinoma cells were inoculated into the liver parenchyma of mice of the model groups by laparotomy .The mice of control group were injected with saline .Fifteen days after tumor cell inoculation , the rate of tumor formation and tumor volume were observed,and the the tumors were examined by pathology .Results The survival rate of mice in all groups was 100%.In the model group I,the tumor transplantation success rate was 100%and the mean tumor volume was （65.21 ±5.25）mm3. In the model group II,the tumor transplantation success rate was 53.3%and the mean tumor volume was （23.46 ±3.47） mm3 .The average tumor volume of the model group I was significantly greater than that of the model group II （ P＆lt;0.05 ） . Pathological examination confirmed hepatocellular carcinoma in the model groups I and II .Conclusion A PLCε gene knockout mouse model of transplanted liver cancer is successfully established , and it demonstrates the property that PLCεgene knockout has an inhibitory effect on liver tumor growth .

关 键 词：基因敲除 PLC8 移植性肝癌 模型 小鼠 

分 类 号：R33[医药卫生—人体生理学]