长爪沙鼠的生物净化技术  

Biological cleaning technologies for Mongolian gerbils

作　　者：卢领群[1] 宋晓明[2] 戴方伟[1] 周莎桑[1] 李长龙[3] 马伟锋[1] 柯贤福[1] 楼琦[1] 吕宇[1] 萨晓婴[1] 

机构地区：[1]浙江省医学科学院实验动物中心,杭州310013 [2]杭州师范大学实验动物中心,杭州310036 [3]首都医科大学基础医学院实验动物学系,北京100069

出　　处：《中国比较医学杂志》2014年第4期62-66,共5页Chinese Journal of Comparative Medicine

基　　金：科技部"十二五"科技支撑计划项目子项目(2013BAK11B01-41);卫生部科研基金(WKJ2010-2-002);浙江省科技计划项目(2012C37097;2011F30009)

摘　　要：目的实现长爪沙鼠微生物质量升级和实验动物化目标,需对长爪沙鼠实施生物净化。方法在长爪沙鼠生物净化过程中,采取孕鼠选择和临产期判断、无菌剖宫产、仔鼠代乳以及独立通风笼盒(IVC)饲养管理等措施,比较了ICR小鼠、SD大鼠、长爪沙鼠代乳和饲料中添加营养成分对仔鼠代乳的影响。结果本研究共实施长爪沙鼠无菌剖宫产达85胎次,通过ICR代乳鼠代乳并成功断乳167只。结论本研究顺利实现了长爪沙鼠生物净化,形成的关键技术有望为我国特色实验动物资源的生物净化提供参考。Objective To obtain higher microbiological quality of Mongolian gerbil colonies and realization of experimental animalization by biological cleaning .Methods We conducted biological cleaning of Mongolian gerbils by selection of pregnant gerbils , perinatal judgment , aseptic Caesarean section , pup milk replacer and breeding in individually ventilated cages .We also compared the results by milk replacer for pup gerbils from lactating ICR mice , rats as well as gerbils.Results During the present study , 85 Caesarean operations were carried out and a total of 167 gerbils were weaned.Conclusions We have successfully achieved the biological cleaning from a Mongolian gerbil colony in Zhejiang province , and the key techniques may provide useful reference for biological cleaning of special laboratory animal species in China.

关 键 词：长爪沙鼠 生物净化 微生物 

分 类 号：R33[医药卫生—人体生理学]