稳定两性离子表面的构筑及其抗蛋白质吸附性能  

Preparation and protein-resistance property of stable zwitterionic surface

作　　者：高娜[1] 李悦[1] 张琴[1] 贾鹏翔[1] 史素青[1] 宫永宽[1] 

机构地区：[1]西北大学合成与天然功能分子化学教育部重点实验室/化学与材料科学学院,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2014年第2期231-236,共6页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(21004047);陕西省教育厅专项科研计划基金资助项目(09JK767);陕西省自然科学基础研究计划基金资助项目(2010JQ2004);教育部博士点新教师基金资助项目(20106101120005)

摘　　要：通过溶胀-锚定(swelling-anchoring)法,用含刚性疏水胆固醇侧基的磷酰胆碱基二元无规共聚物(PMC)构筑稳定两性离子表面涂层,并以含柔性疏水十二烷基酯侧基的磷酰胆碱基二元无规共聚物(PML)为参照聚合物。以水接触角为考察指标对浸涂工艺进行优化。结果发现,丙酮中溶胀5 s后,迅速在质量分数为0.2%PMC73聚合物溶液中浸涂2 h,所得磷酰胆碱两性离子聚合物涂层s-PMC-PC的亲水性最佳,XPS测试结果表明,表面N和P含量分别为2.50%和2.72%。该涂层经过100℃加热2 h和空气中放置2个月处理,接触角基本不变,说明涂层表面稳定性优异;经过75%乙醇中浸泡6 h,1%SDS超声15 min处理,接触角虽然有所升高,但仍远低于对照聚合物涂层,说明该涂层界面附着力有所提高。s-PMC-PC涂层表面抵抗非特异性蛋白质吸附性能优异,Fg和BSA蛋白吸附量可分别降低91.5%和90.8%。A stable zwitterionic surface was fabricated by a simple swelling-anchoring method with phosphorylcholine-based amphiphilic copolymer （PMC） containing rigid hydrophobic cholesteryl side group.The phosphorylcholine-based amphiphilic copolymer （PML） containing flexible alkyl side group was used as control.The influence of types of swelling solvents,swelling time and dip-coating time on the hydrophilicity of the modified PC plate was studied.The results showed that the optimal hydrophilicity could be achieved under the following conditions：acetone as swelling agent,5s of swelling time,0.2wt％ of amphiphilic copolymer and 2hof dip-coating time.XPS test results showed that the surface N and P contents of the coating surface were 2.50％ and 2.72％ respectively.When the coating was heated at 100℃ for 2h or stored in the air for 2 months,the contact angle had no changes.After the coating was soaked in 75％ ethanol for 6 h or by 1％ SDS ultrasonic for 15 min,although the contact angle was increased,s-PMC-PC had still relatively better surface stability and improved interfacial stability.The Fg and BSA protein adsorption on the s-PMC-PC surface had been reduced by 91.5％ and 91.5％ respectively.

关 键 词：两性离子表面 刚性疏水基团 亲水性 蛋白质吸附 

分 类 号：O631[理学—高分子化学]