枣仁安神颗粒中主要成分在大鼠体内的药动学研究  

The pharmacokinetics of the Main Ingredient in Zaoren-an-shen Granule in Rats

作　　者：李珊[1] 王晓锋[1] 党悦方[1] 田娇[1] 赵新锋[1] 王世祥[1] 郑晓晖[1] 房敏峰[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2014年第2期252-255,共4页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(J1210063);陕西省重点科技创新团队计划(2013KCT-24)

摘　　要：建立大鼠血浆中枣仁安神颗粒有效成分斯皮诺素、丹参素钠和五味子醇甲同时测定的高效液相色谱方法.乙腈沉淀法制备血浆样品,C18-反相色谱梯度洗脱法分离斯皮诺素、丹参素钠和五味子醇,内标曲线法计算3种有效成分含量.斯皮诺素、丹参素钠和五味子醇甲的线性范围分别为10 ～300,1.25 ～62.5和5.0～250 μg/mL.3种有效成分日内和日间测定相对标准偏差均小于15％,在低、中、高3个浓度样品回收率均大于85％.正常大鼠灌胃枣仁安神颗粒后,斯皮诺素药代动力学过程为一室模型、丹参素钠和五味子醇的药代动力学过程均呈二室开放模型.该方法能同时测定血浆样品中斯皮诺素、丹参素钠和五味子醇甲,有望作为枣仁安神颗粒的药代动力学研究方法.To establish HPLC method for simultaneously determination of the active ingredients in Zaoren-anshen granule （spinosa,salvianic acid A sodium,schisandrin） in rats.Acetonitrile precipitation preparation of plasma samples,C18 reversed-phase chromatography gradient elution method and separation pinosa,salvianic acid a sodium,schisandrin,internal standard curve method were used to calculate three kinds of effective component content.Three indicators composition was maintained at the linear ranges （10 ～ 300μg/mL for spinosin,1.25 ～62.5 μg/mL for salvianic acid A sodium,5.0 ～250 μg/mlμg/mL for schisandrin）.Three effective constituents measured in daytime and over days showed that the of relative standard deviation was less than 15％.The recovery rates of the samples with low,medium and high concentration were higher than 85％.Normal rats after oral administration Zaoren-an-shen granule,their spinosin conformed to the one-compertment model,both of salvianic acid A and schisandrin conformed to the two-compertment open model.This method can simultaneously determine spinosa,salvianic acid A sodium and schisandrin in plasma samples,can be expected to become a method to research the pharmacokinetic of Zaoren-an-shen granule.

关 键 词：枣仁安神颗粒 斯皮诺素 丹参素钠 五味子醇甲 药动学 

分 类 号：R969.1[医药卫生—药理学]