龙竹半纤维素的提取分离及结构表征  

Isolation and Characterization of Hemicelluloses from Bamboo(Dendrocalamus giganteus Munro)

作　　者：肖霄[1] 李居南 王志文[3] 於祥 徐晖[1] 

机构地区：[1]西北农林科技大学理学院,陕西杨凌712100 [2]中粮营养健康研究院品牌食品研发中心,北京102209 [3]西北农林科技大学林学院,陕西杨凌712100

出　　处：《西北农业学报》2014年第5期195-201,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家重点基础研究发展计划(973计划)(973;2010CB732204)

摘　　要：龙竹竹粉经脱蜡及脱淀粉后,再经亚氯酸钠脱木素制得综纤维素,进一步用二甲亚砜(DMSO)以及KOH溶液提取,经乙醇沉淀后制得半纤维素H1和H2,得率分别为18.7%和39.8%。对半纤维素组分H1和H2进行糖分析、红外光谱以及核磁共振分析。结果表明,龙竹半纤维素为乙酰化的L-阿拉伯糖基-4-O-甲基葡萄糖醛酸木聚糖,其中阿拉伯糖连接在木聚糖主链的C-3位,4-O-甲基葡萄糖醛酸连接在木聚糖主链的C-2位,而乙酰基连接在木聚糖主链的C-2位或C-3位。Bamboo（Dendrocalamus giganteus Munro）powder was dewaxed with toluene-ethanol and destarched with hot water.The extractive free sample was delignified by NaClO2and then extracted with DMSO and KOH,yielding 18.7%（H1）and 39.8%（H2）hemicellulosic fractions,respectively. Chemical composition and structural characterization of these hemicellulosic fractions were investigated by a combination of HPAEC,FT-IR,1 H-,13C-and HSQC NMR techniques.The result revealed that hemicelluloses from bamboo（Dendrocalamus giganteus Munro）were assumed to L-arabino-4-Omethyl-D-glucurono-D-xylan with arabinose linked on position 3of xylan,4-O-methylglucuronic acid residues linked on position 2of the xylan,and acetyl groups linked on position 2or 3.

关 键 词：半纤维素 二甲亚砜 结构表征 核磁共振 

分 类 号：TQ352.1[化学工程]