山东沿海菲律宾蛤仔不同地理群体遗传多样性的AFLP分析  

Analysis of genetic diversity of different geographical populations of Ruditapes philippenarum along the coast of Shandong Province by AFLP markers

作　　者：邹琰[1] 李莉[1] 张良[2] 吕芳[1] 辛美丽[1] 张少春[1] 邱兆星[1] 刘洪军[1] 

机构地区：[1]山东省海水养殖研究所山东省海水健康养殖工程技术研究中心,山东青岛266002 [2]国家海洋局第一海洋研究所,山东青岛266061

出　　处：《海洋科学》2014年第2期76-79,共4页Marine Sciences

基　　金：山东省良种工程课题"蛤类优质高产抗逆贝类选育"与"水产经济生物种质资源收集;保护与评价"联合资助

摘　　要：利用AFLP技术对山东沿海菲律宾蛤仔（Ruditapes philippinarum）4个野生群体（乳山、无棣、牟平、即墨）进行了遗传多样性分析。采用6对引物组合对4个群体进行扩增,共得到356个位点,乳山群体、无棣群体、牟平群体和即墨群体的多态位点比例依次为74.40%、70.50%、72.80%、74.70%,具有较高的遗传多样性水平。其中,即墨群体多态性比例最高,无棣群体多态性比例最低。聚类分析表明,各群体间的遗传距离为0.0263-0.0448,乳山群体和无棣群体间的遗传距离最近（0.0263）,两群体首先聚在一起,随后与牟平群体和即墨群体聚在一起。The AFLP technique was applied to investigate genetic diversity of 4 geographical populations of Ruditapes philippinarum in coastal sea of Shandong. Six primer pairs were used to generate 356 loci among individuals in 4 populations. The proportion of polymorphic loci for the Rushan, Wudi, Muping and Jimo populaton was 74.40%, 70.50%, 72.80%and 74.70% respectively, suggesting high levels of genetic diversity. The Jimo population exhibited the highest genetic diversity, and the Wudi population exhibted the lowest one. The cluster analysis showed that the genetic distance among the 4 populations was 0.0263-0.0448 and the genetic distance between the Rushan and Wudi population was the smallest one （0.0263）. The Rushan and Wudi populations were clustered together and subsequently grouped with Muping-Jimo clade.

关 键 词：菲律宾蛤仔(Ruditapes philippinarum) 地理群体 遗传多样性 

分 类 号：S917.4[农业科学—水产科学]