GSN antibody pretreatment aggravates radiation-induced lung injury in mice  

作　　者：王洁 袁小鹏 顾成 黄建峰 俞家华 刘芬菊 

机构地区：[1]Department of Radiobiology,School of Radiation Medicine and Protection,Medical College of Soochow University [2]School for Radiological and Interdisciplinary Sciences,Soochow University

出　　处：《Nuclear Science and Techniques》2014年第2期26-31,共6页核技术（英文）

基　　金：Supported by grants from the National Natural Science Foundation of China(No.31270897,81202149,and 81271682);Graduate Education Innovation Project of Jiangsu Province and the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)

摘　　要：Radiation-induced lung injury is one of the main dose limiting factors for thoracic radiation therapy.Gelsolin(GSN) is a widespread,multifunctional regulator of cellular structure and metabolism.In this work,the roles of GSN in radiation-induced lung injury in Balb/c mice were studied.The GSN levels in plasma reduced progressively in 72 hours after irradiation,and then increased gradually.GSN contents in the bronchoalveolar lavage(BAL) fluid increased after thoracic irradiation,whereas mRNA levels of GSN in the lung tissue decreased significantly within 24 hours after irradiation and then increased again.Mice were intravenously injected with 50 ug GSN antibody 0.5 hour before 20 Gy of thoracic irradiation.GSN antibody pretreatment increased lung inflammation,protein concentration in the BAL fluid and leukocytes infiltration in the irradiated mice.The activities of superoxidase dismutase(SOD) in the plasma and the BAL fluid in irradiated mice injected with GSN antibody were less than that of control groups,whereas the levels of malondialdehyde(MDA)increased.These results suggest that pretreatment of GSN antibody may aggravate radiation-induced pneumonitis.Radiation-induced lung injury is one of the main dose limiting factors for thoracic radiation therapy. Gel- solin （GSN） is a widespread, multifunctional regulator of cellular structure and metabolism. In this work, the roles of GSN in radiation-induced lung injury in Balb/c mice were studied. The GSN levels in plasma reduced progressively in 72 hours after irradiation, and then increased gradually. GSN contents in the bronchoalve- olar lavage （BAL） fluid increased after thoracic irradiation, whereas mRNA levels of GSN in the lung tissue decreased significantly within 24 hours after irradiation and then increased again. Mice were intravenously injected with 50 lag GSN antibody 0.5 hour before 20 Gy of thoracic irradiation. GSN antibody pretreatment increased lung inflammation, protein concentration in the BAL fluid and leukocytes infiltration in the irradiated mice. The activities of superoxidase dismutase （SOD） in the plasma and the BAL fluid in irradiated mice in- jected with GSN antibody were less than that of control groups, whereas the levels of malondialdehyde （MDA） increased. These results suggest that pretreatment of GSN antibody may aggravate radiation-induced pneumoni- tis.

关 键 词：GSN 肺损伤 放射性 预处理 小鼠 抗体 MRNA水平 超氧化物歧化酶 

分 类 号：R818[医药卫生—放射医学]