人参皂苷M1异丁酸酯衍生物的合成及高效液相分析和制备  

Synthesis of Ginsenoside M1 Isobutyrate and its Analysis and Preparation by HPLC

作　　者：肖景楠[1] 李珂珂[2] 李争宁[1] 陈丽荣[1] 弓晓杰[2] 

机构地区：[1]大连大学环境与化学工程学院,辽宁大连116622 [2]大连大学医学院,辽宁大连116622

出　　处：《中国现代中药》2014年第5期367-371,共5页Modern Chinese Medicine

基　　金：国家自然科学基金(81172949);辽宁省教育厅科学技术研究一般研究项目(L2012439);辽宁省优秀人才支持计划(LR2013058);辽宁省科技厅科学技术计划项目(2013204001);大连市科技局科技计划项目(2013E11SF055)

摘　　要：目的:研究人参皂苷M1异丁酸酯合成过程中产物的高效液相分析及快速制备方法。方法:采用保护-酯化-去保护的方法,定向修饰得到M1糖基3-位酯化的产物;利用反相高效液相色谱,采用DAD检测器,通过对分析条件的优化,建立人参皂苷M1异丁酸酯及其两个中间产物的分析制备方法。结果:人参皂苷M1异丁酸酯及其中间产物的分析和制备时间均在40 min以内快速高效地获得;综合三步反应和高效液相制备结果,合成人参皂苷M1异丁酸酯的得率为36.7%,纯度为98.3%。结论:采用HPLC法,在M1衍生物合成过程中可以快速高效地进行人参皂苷M1衍生物的分析和制备。Objective： Ginsenoside M1 isobutyrate and the intermediate product in the process of synthesis were analyzed and prepared by HPLC. Methods： Using the protection esterification-deprotection strategy,ginsenoside M1 3-sugar residues esterification product was synthesed. The analysis and preparation method of M1 isobutyrate and the intermediate product by RP-HPLC-DAD was established and optimized. Results： The analysis time of ginsenoside M1 isobutyrate and the intermediate product was all less than 40 minutes. Taking all the three steps of synthesis into account,the yield was 36. 7%,and purity was 98. 3%. Conclusion： The HPLC method put forward a rapid and efficient strategy to deal with the products through the process of ginsenoside M1 synthesis.

关 键 词：人参皂苷M1 结构修饰 高效液相 含量分析 

分 类 号：R516.1[医药卫生—内科学]